Overview

  • Product name
  • Description
    Rabbit polyclonal to groEL
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    E. coli groEL protein

  • Positive control
    • WB: Recombinant groEL protein; E.coli cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab90522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 57 kDa.

Target

  • Relevance
    The bacterial chaperonin groEL is a double toroidal assembly, which together with the action of the ring-shaped oligomeric cochaperonin, GroES, facilitates protein folding in an ATP dependent manner.
  • Cellular localization
    Cytoplasmic
  • Database links
    • Alternative names
      • 60 kDa chaperonin antibody
      • groEL protein antibody
      • groL antibody
      • mopA antibody
      • Protein Cpn60 antibody
      see all

    Images

    • All lanes : Anti-groEL antibody (ab90522) at 1/1000 dilution

      Lane 1 : groEL recombinant protein
      Lane 2 : Human recombinant HSP60
      Lane 3 : Heat Shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
      Lane 4 : E.coli cell lysate

      Predicted band size: 57 kDa

    References

    This product has been referenced in:
    • Sugimoto S  et al. Selective growth inhibition of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans by antisense peptide nucleic acids. Mol Cell Probes 43:45-49 (2019). Read more (PubMed: 30471338) »
    • Eckert CA  et al. Inactivation of the uptake hydrogenase in the purple non-sulfur photosynthetic bacterium Rubrivivax gelatinosus CBS enables a biological water-gas shift platform for H2 production. J Ind Microbiol Biotechnol N/A:N/A (2019). Read more (PubMed: 30968274) »
    See all 7 Publications for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Application
    Western blot
    Sample
    Pseudomonas aeruginosa Cell lysate - whole cell (pseudomonas aeruginosa)
    Gel Running Conditions
    Non-reduced Denaturing
    Loading amount
    1e+006 cells
    Specification
    pseudomonas aeruginosa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Yu Zhang

    Verified customer

    Submitted Jan 12 2016

    Application
    Western blot
    Loading amount
    75 µg
    Gel Running Conditions
    Reduced Denaturing (18)
    Sample
    Escherichia coli Cell lysate - whole cell (E. coli Deletion variants)
    Specification
    E. coli Deletion variants
    Treatment
    20 mM Nitrat, no oxygen for 16 hrs
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Oct 14 2014

    Answer


    I am very pleased to hear you would like to accept our offer and test ab90522 in P. aerugionsa. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for P. aerugionsa and include this code in the Additional Comments section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.


    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Question
    Answer

    Thank you very much for your interest in ab90522.

    To our knowledge, ab90522 has not been tested in P. aerugionsa. Therefore, I can offer a discount off a future purchase if you buy ab90522 now, test it in P. aerugionsa and submit feedback to us in the form of an Abreview. It doesn't matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.
    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab90522 in P. aerugionsa. I will then send a discount code. This code must be issued before purchasing ab90522 so please wait for my reply before ordering.

    2. Purchase ab90522 either by phone, fax, or online (www.abcam.com).

    3. Test it in P. aerugionsa.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab90522 turns out to be unsuitable for P. aerugionsa, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Answer

    Thank you for contacting Abcam regarding ab90522.


    We have not tested this cross reactivity specifically so I did an alignment. Based on a sequence alignment in Uniprot, there is only about 60% homology between various strains of E. Coli GroEL and streptococcus pneumoniae GroEL so unfortunately there is not a chance of cross reactivity.


    I am sorry for any inconvenience this may cause. Please do not hesitate to contact me if you have any additional questions.

    Read More

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab82592.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

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