Anti-groEL antibody [9A1/2] (ab82592)
Key features and details
- Mouse monoclonal [9A1/2] to groEL
- Suitable for: WB, IP
- Reacts with: Escherichia coli
- Isotype: IgG1
Overview
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Product name
Anti-groEL antibody [9A1/2]
See all groEL primary antibodies -
Description
Mouse monoclonal [9A1/2] to groEL -
Host species
Mouse -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Full length protein corresponding to Escherichia coli groEL. Strain K12
Database link: P0A6F5 -
Positive control
- E. coli lysate.
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General notes
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
9A1/2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab82592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
IP
Use at an assay dependent concentration. |
Target
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Relevance
The bacterial chaperonin groEL is a double toroidal assembly, which together with the action of the ring-shaped oligomeric cochaperonin, GroES, facilitates protein folding in an ATP dependent manner. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 1037522 Escherichia coli
- SwissProt: P0A6F5 Escherichia coli
- SwissProt: P0A6F6 Escherichia coli
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Alternative names
- 60 kDa chaperonin antibody
- groEL protein antibody
- groL antibody
see all
Images
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All lanes : Anti-groEL antibody [9A1/2] (ab82592) at 1/1000 dilution
Lane 1 : groEL recombinant E. coli protein
Lane 2 : Hsp60 recombinant human protein (negative control)
Lane 3 : E. coli lysate
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 57 kDaThis image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab82592 has been referenced in 18 publications.
- Fang J et al. Mycobacterium tuberculosis Rv0790c inhibits the cellular autophagy at its early stage and facilitates mycobacterial survival. Front Cell Infect Microbiol 12:1014897 (2022). PubMed: 36439208
- Yeom J & Groisman EA Low Cytoplasmic Magnesium Increases the Specificity of the Lon and ClpAP Proteases. J Bacteriol 203:e0014321 (2021). PubMed: 33941609
- Bao SH et al. A dynamic and multilocus metabolic regulation strategy using quorum-sensing-controlled bacterial small RNA. Cell Rep 36:109413 (2021). PubMed: 34289355
- Schuller M et al. Molecular basis for DarT ADP-ribosylation of a DNA base. Nature 596:597-602 (2021). PubMed: 34408320
- Iosub IA et al. Hfq CLASH uncovers sRNA-target interaction networks linked to nutrient availability adaptation. Elife 9:N/A (2020). PubMed: 32356726