Anti-Growth Hormone antibody (ab126882)

Rabbit polyclonal Growth Hormone antibody. Validated in WB, IP, ELISA, IHC and tested in Rat. Independently reviewed in 2 review(s). Immunogen corresponding to recombinant full length protein.

Overview

  • Product name

    Anti-Growth Hormone antibody
    See all Growth Hormone primary antibodies
  • Description

    Rabbit polyclonal to Growth Hormone
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat
  • Immunogen

    Recombinant full length mature protein, corresponding to amino acids 27-216 of Rat Growth Hormone, derived from E.coli.

Properties

Applications

Our Abpromise guarantee covers the use of ab126882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. 0.5µg (0.5ng/lane)
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration. Use 0.2ng/well
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
  • Involvement in disease

    Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
    Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
    Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
    Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy.
  • Sequence similarities

    Belongs to the somatotropin/prolactin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • gH antibody
    • GH-N antibody
    • GH1 antibody
    • GHB5 antibody
    • GHN antibody
    • Growth hormone 1 antibody
    • Growth hormone antibody
    • Growth hormone B5 antibody
    • Growth hormone, normal antibody
    • Growth hormone, pituitary antibody
    • HG1 antibody
    • hGH-N antibody
    • IGHD1B antibody
    • Pituitary growth hormone antibody
    • RNGHGP antibody
    • SOMA_HUMAN antibody
    • Somatotropin antibody
    see all

References

ab126882 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse pituitary gland tissue)
Permeabilization
Yes - 0.05% saponin in PBS
Specification
Mouse pituitary gland tissue
Blocking step
No blocking step used for 30 minute(s) · Concentration: 0 · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 19 2018

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Sample
Rat Tissue sections (diagonal band)
Specification
diagonal band
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 08 2013

Answer

We have done some preliminary test to make sure that this antibody works but detailed test data is not yet available. Because of that we plan to take off the neutralization application from our datasheet for now and add it back once we have more data. This will be reflected on the datasheet soon.

Read More

Answer

Thank you for your message.

The current availability of each product is provided on the individual datasheets.

For ab30538, for the UK it currently states: 'Order now and get it on Tuesday 27 November 2012'.

So if you place your order by 4 pm today, you would receive it tomorrow. We have a few in stock, but I can still recommend to place your order as soon as possible in case we receive other orders for this product and availability changes.

Regarding long term availability, I can confirm we have no current plans to discontinue this product and it is not likely we will do so in the near future. However, please note that decisions regarding products on the catalog can sometimes be made quickly, and so I am sorry I we are regrettably not able to provide a guarantee of how long this will be available for.

I hope this will be helpful. If you have any further questions please do not hesitate to contact me.

Read More

Question

Hi

Many thanks for your e-mail. You mention that this product has not been tested with samples from mouse – am I right to assume it has not been tested with human, bovine or porcine samples either because these are not mentioned on the datasheet?

Are you also able to tell me what the future supply of this antibody is likely to be? If the product works for our assay we may be looking at fairly regular orders long term so we would run into problems if the product was discontinued or if lead times were to be extended significantly. I know it may be difficult to answer these questions at this stage, but if you're able to give any indication of long term supply I'd be very grateful!

Best wishes,

Polly






Polly Taylor

Senior Scientist, Research Projects

HFL Sport Science

TEL: +44 (1638) 724420 FAX: +44 (1638) 724401

www.hfl.co.uk






From: technical@abcam.com [mailto:technical@abcam.com]
Sent: 26 September 2012 16:59
To: Polly Taylor
Subject: Reply from Abcam to your enquiry regarding ab126882 [CCE4188191]



















https://www.abcam.com/index.html?utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Header









Dear Polly

Thank you for contacting us and for your interest in our products.

Regarding availability, this item is in stock and will be available for next day delivery if ordered before 4 pm in the UK. The availability and delivery time is available at the top of the online datasheet.

As far as we are aware, ab126882 has never been tested with samples from mouse. All tested species covered by our 6 month guarantee are stated on our datasheets, and these are updated as soon as any new information is brought to our attention.

If you would like to test the antibody in this a previouslyuntested species, please do not hesitate to contact me again prior to the purchaseby replying to this message as you may be eligible for our testing discount program.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.




Help us improve our service.
https://www.abcam.com/index.html?pageConfig=technicalSurvey&intCCEID=4188191


Best regards,
Kate

Kate Hayes
Scientific Support Supervisor
Abcam plc
https://www.abcam.com


Your original inquiry to Abcam:


Hi,


I was wondering if you’d be able to tell me what the availability of the above product (ab126882) is currently? Do you know what the likely lead time on this product is for delivery within the UK?

Also, do you have any data regarding the cross-reactivity of this antibody with growth hormone from species other than rat (e.g. human, bovine, porcine etc)?

Many thanks,

Polly Taylor





Polly Taylor

Senior Scientist, Research Projects

HFL Sport Science

TEL: +44 (1638) 724420 FAX: +44 (1638) 724401

http://www.hfl.co.uk





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This email and any attachments are confidential. Any use, copying or
disclosure other than by the intended recipient is unauthorised. If
you have received this message in error, please notify the sender
immediately via +44(0)20 8943 7000 or notify postmaster@lgcgroup.com
and delete this message and any copies from your computer and network.
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Registered office: Queens Road, Teddington, Middlesex, TW11 0LY, UK

Read More
Answer

Thank you for your reply.

I'm sorry I should perhaps have been more specific and clear in my last message and am not sure why I mentioned mouse in particular.

You are right, only tested species will be listed on the datasheet. If a species has been tested and the results have not been successful, it will be listed as 'does not react with'.

Therefore, this antibody (ab126882 Anti-Rat Growth Hormone antibody) has not been tested in human, cow or pig.

We recommend that alignment with the immunogen should be over 85% to predict that an antibody will detect in a different species. In this case, the alignment with the species you are interested in are as follows:


Human 65%
Bovine 87%
Pig 94%

Therefore, I would suggest that the antibody will detect in bovine and pig samples as the alignment is high, although please note we are not able to guarantee this without further testing. For human, the alignment is lower, and at 65% there may be some crossreactivity although itis likelynot togive a verystrong signal.

I hope this will be helpful information for you. If you would like to test ab126882 in cow or pig, please do not hesitate to contact me again prior to the purchaseby replying to this message as you may be eligible for our testing discount program and I'll be pleased to send you more information.

With regards to availability of this product, I am sorry we are not able to guarantee the availability of any of our products in the long term. However, I have spoken to our business development commercial department, who have assured me that we have no current plans to discontinue this antibody. However, this can change quite quickly, particularly for items that are sources externally,so please note we cannot guarantee this.

I hope this information will beuseful. If you have any further questions, please do not hesitate to contact us.

Read More

Answer

Thank you for contacting us and for your interest in our products.

Regarding availability, this item is in stock and will be available for next day delivery if ordered before 4 pm in the UK. The availability and delivery time is available at the top of the online datasheet.

As far as we are aware, ab126882 has never been tested with samples from mouse. All tested species covered by our 6 month guarantee are stated on our datasheets, and these are updated as soon as any new information is brought to our attention.

If you would like to test the antibody in this a previouslyuntested species, please do not hesitate to contact me again prior to the purchaseby replying to this message as you may be eligible for our testing discount program.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

Read More

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