Anti-Growth Hormone antibody (ab126882)

Reviews (2)Q&A (4)References (1)

Key features and details

  • Rabbit polyclonal to Growth Hormone
  • Reacts with: Rat
  • Isotype: IgG

Overview

  • Product name

    Anti-Growth Hormone antibody
    See all Growth Hormone primary antibodies

  • Description

    Rabbit polyclonal to Growth Hormone

  • Host species

    Rabbit

  • Species reactivity

    Reacts with: Rat

  • Immunogen

    Recombinant full length mature protein, corresponding to amino acids 27-216 of Rat Growth Hormone, derived from E.coli.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Target

  • Function

    Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

  • Involvement in disease

    Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
    Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
    Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
    Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy.

  • Sequence similarities

    Belongs to the somatotropin/prolactin family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • gH antibody
      • GH-N antibody
      • GH1 antibody
      • GHB5 antibody
      • GHN antibody
      • Growth hormone 1 antibody
      • Growth hormone antibody
      • Growth hormone B5 antibody
      • Growth hormone, normal antibody
      • Growth hormone, pituitary antibody
      • HG1 antibody
      • hGH-N antibody
      • IGHD1B antibody
      • Pituitary growth hormone antibody
      • RNGHGP antibody
      • SOMA_HUMAN antibody
      • Somatotropin antibody
      see all

    References (1)

    Publishing research using ab126882? Please let us know so that we can cite the reference in this datasheet.

    ab126882 has been referenced in 1 publication.

    • Alshafie W  et al. Regulated resurfacing of a somatostatin receptor storage compartment fine-tunes pituitary secretion. J Cell Biol 219:N/A (2020). PubMed: 31825461

    Customer reviews and Q&As

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    1-6 of 6 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Growth Hormone antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mouse pituitary gland tissue)
    Permeabilization
    Yes - 0.05% saponin in PBS
    Specification
    Mouse pituitary gland tissue
    Blocking step
    No blocking step used for 30 minute(s) · Concentration: 0 · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 19 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-Rat Growth Hormone antibody

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
    Sample
    Rat Tissue sections (diagonal band)
    Specification
    diagonal band
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 08 2013

    Question

    The antibody ab126882 is listed as being a neutralizing Ab for rat growth hormone. Can you provide details on how the neutralizing activity was tested and data showing its effectiveness?

    Read More
    Answer

    We have done some preliminary test to make sure that this antibody works but detailed test data is not yet available. Because of that we plan to take off the neutralization application from our datasheet for now and add it back once we have more data. This will be reflected on the datasheet soon.

    Read More

    Question

    Hi,

    I was wondering if you could give me an idea of the lead time on ab30538 (mouse anti-bovine growth hormone monoclonal antibody)? Are you also able to tell me what the mid- to long-term supply of this antibody is likely to be like? Are there any current plans to discontinue this antibody?

    Many thanks,

    Read More
    Answer

    Thank you for your message.

    The current availability of each product is provided on the individual datasheets.

    For ab30538, for the UK it currently states: 'Order now and get it on Tuesday 27 November 2012'.

    So if you place your order by 4 pm today, you would receive it tomorrow. We have a few in stock, but I can still recommend to place your order as soon as possible in case we receive other orders for this product and availability changes.

    Regarding long term availability, I can confirm we have no current plans to discontinue this product and it is not likely we will do so in the near future. However, please note that decisions regarding products on the catalog can sometimes be made quickly, and so I am sorry I we are regrettably not able to provide a guarantee of how long this will be available for.

    I hope this will be helpful. If you have any further questions please do not hesitate to contact me.

    Read More

    Question

    Hi

    Many thanks for your e-mail. You mention that this product has not been tested with samples from mouse – am I right to assume it has not been tested with human, bovine or porcine samples either because these are not mentioned on the datasheet?

    Are you also able to tell me what the future supply of this antibody is likely to be? If the product works for our assay we may be looking at fairly regular orders long term so we would run into problems if the product was discontinued or if lead times were to be extended significantly. I know it may be difficult to answer these questions at this stage, but if you're able to give any indication of long term supply I'd be very grateful!

    Best wishes,

    Polly






    Polly Taylor

    Senior Scientist, Research Projects

    HFL Sport Science

    TEL: +44 (1638) 724420 FAX: +44 (1638) 724401

    www.hfl.co.uk






    From: technical@abcam.com [mailto:technical@abcam.com]
    Sent: 26 September 2012 16:59
    To: Polly Taylor
    Subject: Reply from Abcam to your enquiry regarding ab126882 [CCE4188191]



















    https://www.abcam.com/index.html?utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Header









    Dear Polly

    Thank you for contacting us and for your interest in our products.

    Regarding availability, this item is in stock and will be available for next day delivery if ordered before 4 pm in the UK. The availability and delivery time is available at the top of the online datasheet.

    As far as we are aware, ab126882 has never been tested with samples from mouse. All tested species covered by our 6 month guarantee are stated on our datasheets, and these are updated as soon as any new information is brought to our attention.

    If you would like to test the antibody in this a previouslyuntested species, please do not hesitate to contact me again prior to the purchaseby replying to this message as you may be eligible for our testing discount program.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.




    Help us improve our service.
    https://www.abcam.com/index.html?pageConfig=technicalSurvey&intCCEID=4188191


    Best regards,
    Kate

    Kate Hayes
    Scientific Support Supervisor
    Abcam plc
    https://www.abcam.com


    Your original inquiry to Abcam:


    Hi,


    I was wondering if you’d be able to tell me what the availability of the above product (ab126882) is currently? Do you know what the likely lead time on this product is for delivery within the UK?

    Also, do you have any data regarding the cross-reactivity of this antibody with growth hormone from species other than rat (e.g. human, bovine, porcine etc)?

    Many thanks,

    Polly Taylor





    Polly Taylor

    Senior Scientist, Research Projects

    HFL Sport Science

    TEL: +44 (1638) 724420 FAX: +44 (1638) 724401

    http://www.hfl.co.uk





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    *******************************************************************
    This email and any attachments are confidential. Any use, copying or
    disclosure other than by the intended recipient is unauthorised. If
    you have received this message in error, please notify the sender
    immediately via +44(0)20 8943 7000 or notify postmaster@lgcgroup.com
    and delete this message and any copies from your computer and network.
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    Registered office: Queens Road, Teddington, Middlesex, TW11 0LY, UK

    Read More
    Answer

    Thank you for your reply.

    I'm sorry I should perhaps have been more specific and clear in my last message and am not sure why I mentioned mouse in particular.

    You are right, only tested species will be listed on the datasheet. If a species has been tested and the results have not been successful, it will be listed as 'does not react with'.

    Therefore, this antibody (ab126882 Anti-Rat Growth Hormone antibody) has not been tested in human, cow or pig.

    We recommend that alignment with the immunogen should be over 85% to predict that an antibody will detect in a different species. In this case, the alignment with the species you are interested in are as follows:


    Human 65%
    Bovine 87%
    Pig 94%

    Therefore, I would suggest that the antibody will detect in bovine and pig samples as the alignment is high, although please note we are not able to guarantee this without further testing. For human, the alignment is lower, and at 65% there may be some crossreactivity although itis likelynot togive a verystrong signal.

    I hope this will be helpful information for you. If you would like to test ab126882 in cow or pig, please do not hesitate to contact me again prior to the purchaseby replying to this message as you may be eligible for our testing discount program and I'll be pleased to send you more information.

    With regards to availability of this product, I am sorry we are not able to guarantee the availability of any of our products in the long term. However, I have spoken to our business development commercial department, who have assured me that we have no current plans to discontinue this antibody. However, this can change quite quickly, particularly for items that are sources externally,so please note we cannot guarantee this.

    I hope this information will beuseful. If you have any further questions, please do not hesitate to contact us.

    Read More

    Question

    Hi,

    I was wondering if you’d be able to tell me what the availability of the above product (ab126882) is currently? Do you know what the likely lead time on this product is for delivery within the UK?

    Also, do you have any data regarding the cross-reactivity of this antibody with growth hormone from species other than rat (e.g. human, bovine, porcine etc)?

    Many thanks,

    Read More
    Answer

    Thank you for contacting us and for your interest in our products.

    Regarding availability, this item is in stock and will be available for next day delivery if ordered before 4 pm in the UK. The availability and delivery time is available at the top of the online datasheet.

    As far as we are aware, ab126882 has never been tested with samples from mouse. All tested species covered by our 6 month guarantee are stated on our datasheets, and these are updated as soon as any new information is brought to our attention.

    If you would like to test the antibody in this a previouslyuntested species, please do not hesitate to contact me again prior to the purchaseby replying to this message as you may be eligible for our testing discount program.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

    Read More

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