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RecombinantRabMAb

Recombinant Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)

Submit a review Submit a question References (2)
Western blot - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)
  • Immunoprecipitation - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)
  • Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11047(B)] to Growth Hormone
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Growth Hormone antibody [EPR11047(B)]
    See all Growth Hormone primary antibodies

  • Description

    Rabbit monoclonal [EPR11047(B)] to Growth Hormone

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Growth Hormone aa 50-150. The exact sequence is proprietary.
    Database link: P01241

  • Positive control

    • WB: Human placenta lysate. IHC: Human placenta and Human pituitary gland tissue. IP: Human placenta lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.
Application notes
Is unsuitable for ICC.

Target

  • Function

    Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

  • Involvement in disease

    Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
    Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
    Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
    Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy.

  • Sequence similarities

    Belongs to the somatotropin/prolactin family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • gH antibody
      • GH-N antibody
      • GH1 antibody
      • GHB5 antibody
      • GHN antibody
      • Growth hormone 1 antibody
      • Growth hormone antibody
      • Growth hormone B5 antibody
      • Growth hormone, normal antibody
      • Growth hormone, pituitary antibody
      • HG1 antibody
      • hGH-N antibody
      • IGHD1B antibody
      • Pituitary growth hormone antibody
      • RNGHGP antibody
      • SOMA_HUMAN antibody
      • Somatotropin antibody
      see all

    Images

    • Western blot - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)
      Western blot - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)
      Anti-Growth Hormone antibody [EPR11047(B)] (ab155276) at 1/1000 dilution + Human placenta lysate at 10 µg

      Predicted band size: 25 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)

      Immunohistochemical analysis of paraffin embedded human pituitary gland tissue labeling Human Growth Hormone with ab155276 at a 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)

      Immunohistochemical analysis of paraffin embedded Human placenta tissue using ab155276 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)
      Immunoprecipitation - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)

      Growth Hormone was immunoprecipitated from 0.35 mg Human placenta lysate 10 µg with 155276 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Human placenta lysate 10 µg

      Lane 2: ab155276 IP in Human placenta lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab155276 Human placenta lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)

      Immunohistochemical analysis of paraffin embedded Human normal tonsil tissue using ab155276 showing -ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)

      Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab155276 showing -ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)

      Immunohistochemical analysis of paraffin embedded Human heart tissue using ab155276 showing -ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)

      Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue using ab155276 showing -ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)
      Anti-Growth Hormone antibody [EPR11047(B)] (ab155276)

    References (2)

    Publishing research using ab155276? Please let us know so that we can cite the reference in this datasheet.

    ab155276 has been referenced in 2 publications.

    • Chen Y  et al. Functional characterization of DLK1/MEG3 locus on chromosome 14q32.2 reveals the differentiation of pituitary neuroendocrine tumors. Aging (Albany NY) 13:1422-1439 (2020). PubMed: 33472171
    • Basu R  et al. Targeting growth hormone receptor in human melanoma cells attenuates tumor progression and epithelial mesenchymal transition via suppression of multiple oncogenic pathways. Oncotarget 8:21579-21598 (2017). WB ; Human . PubMed: 28223541

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