Recombinant Anti-Growth Hormone antibody [EPR9524] (ab155974)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9524] to Growth Hormone
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-Growth Hormone antibody [EPR9524]
See all Growth Hormone primary antibodies -
Description
Rabbit monoclonal [EPR9524] to Growth Hormone -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: ICC or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Growth Hormone.
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Positive control
- Human Growth Hormone recombinant protein; Human pituitary lysate; Human pituitary tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9524 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab155974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 17-22 kDa (predicted molecular weight: 25 kDa). |
Target
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Function
Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. -
Involvement in disease
Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy. -
Sequence similarities
Belongs to the somatotropin/prolactin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2688 Human
- Omim: 139250 Human
- SwissProt: P01241 Human
- Unigene: 655229 Human
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Alternative names
- gH antibody
- GH-N antibody
- GH1 antibody
see all
Images
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![Western blot - Anti-Growth Hormone antibody [EPR9524] (ab155974)](https://www.abcam.com/ps/products/155/ab155974/Images/Human-Growth-Hormone-Primary-antibodies-ab155974-2.jpg)
Western blot - Anti-Growth Hormone antibody [EPR9524] (ab155974)Anti-Growth Hormone antibody [EPR9524] (ab155974) at 1/1000 dilution + Human pituitary lysate at 10 µg
Secondary
HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 17-22 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR9524] (ab155974)](https://www.abcam.com/ps/products/155/ab155974/Images/Human-Growth-Hormone-Primary-antibodies-ab155974-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR9524] (ab155974)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pituitary tissue labeling Human Growth Hormone with ab155974 at 1/100 dilution
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Growth Hormone antibody [EPR9524] (ab155974)](https://www.abcam.com/ps/products/155/ab155974/Images/Human-Growth-Hormone-Primary-antibodies-ab155974-1.jpg)
Western blot - Anti-Growth Hormone antibody [EPR9524] (ab155974)Anti-Growth Hormone antibody [EPR9524] (ab155974) at 1/1000 dilution + Human Growth Hormone recombinant protein with proprietary tag at 0.01 µg
Secondary
HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 17-22 kDa why is the actual band size different from the predicted? -
![Anti-Growth Hormone antibody [EPR9524] (ab155974)](https://www.abcam.com/ps/products/155/ab155974/Images/ab155974-4-benefits-of-recombinant-antibodies.png)
Anti-Growth Hormone antibody [EPR9524] (ab155974)
Protocols
Datasheets and documents
References (0)
ab155974 has not yet been referenced specifically in any publications.
