
Anti-Growth Hormone antibody [EPR9524] (ab155974)
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Overview
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Product nameAnti-Growth Hormone antibody [EPR9524]
See all Growth Hormone primary antibodies -
DescriptionRabbit monoclonal [EPR9524] to Growth Hormone
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: ICC or IP -
Species reactivityReacts with: Human
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Immunogen
Recombinant fragment corresponding to amino acids within Human Growth Hormone (UniProt ID: P01241).
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Positive control
- Human Growth Hormone recombinant protein; Human pituitary lysate; Human pituitary tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR9524
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IsotypeIgG
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Research areas
Associated products
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Alternative Products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab155974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | 1/100 - 1/250. | |
WB | 1/1000 - 1/10000. Detects a band of approximately 17-22 kDa (predicted molecular weight: 25 kDa). |
Target
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FunctionPlays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
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Involvement in diseaseDefects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy. -
Sequence similaritiesBelongs to the somatotropin/prolactin family.
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Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 2688 Human
- Omim: 139250 Human
- SwissProt: P01241 Human
- Unigene: 655229 Human
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Alternative names
- gH antibody
- GH-N antibody
- GH1 antibody
see all
Images
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Anti-Growth Hormone antibody [EPR9524] (ab155974) at 1/1000 dilution + Human pituitary lysate at 10 µg
Secondary
HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 17-22 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR9524] (ab155974)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pituitary tissue labeling Human Growth Hormone with ab155974 at 1/100 dilution
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Anti-Growth Hormone antibody [EPR9524] (ab155974) at 1/1000 dilution + Human Growth Hormone recombinant protein with proprietary tag at 0.01 µg
Secondary
HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 17-22 kDa why is the actual band size different from the predicted?
Datasheets and documents
References
ab155974 has not yet been referenced specifically in any publications.