Overview

  • Product name

    Anti-Growth Hormone antibody [GH-1]
    See all Growth Hormone primary antibodies
  • Description

    Mouse monoclonal [GH-1] to Growth Hormone
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, Indirect ELISA, WB, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

  • Epitope

    Ab9821 recognizes a different epitope than GH-2 (ab9822).
  • General notes

    This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.

Properties

Applications

Our Abpromise guarantee covers the use of ab9821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/250.
Indirect ELISA 1/1000 - 1/10000.
WB 1/500 - 1/2000. Predicted molecular weight: 22-24 kDa.
Sandwich ELISA Use at an assay dependent dilution.

Target

  • Function

    Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
  • Involvement in disease

    Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
    Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
    Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
    Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy.
  • Sequence similarities

    Belongs to the somatotropin/prolactin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • gH antibody
    • GH-N antibody
    • GH1 antibody
    • GHB5 antibody
    • GHN antibody
    • Growth hormone 1 antibody
    • Growth hormone antibody
    • Growth hormone B5 antibody
    • Growth hormone, normal antibody
    • Growth hormone, pituitary antibody
    • HG1 antibody
    • hGH-N antibody
    • IGHD1B antibody
    • Pituitary growth hormone antibody
    • RNGHGP antibody
    • SOMA_HUMAN antibody
    • Somatotropin antibody
    see all

Images

  • Western blot of human growth hormone using ab9821 at a concentration of 1 µg/ml.

    Western blot of human growth hormone using ab9821 at a concentration of 1 µg/ml.

References

This product has been referenced in:

  • Liu Y  et al. Ghrelin protects the myocardium with hypoxia/reoxygenation treatment through upregulating the expression of growth hormone, growth hormone secretagogue receptor and insulin-like growth factor-1, and promoting the phosphorylation of protein kinase B. Int J Mol Med 42:3037-3046 (2018). Read more (PubMed: 30272367) »
  • Sun H  et al. A monoclonal antibody against KCNK9 K(+) channel extracellular domain inhibits tumour growth and metastasis. Nat Commun 7:10339 (2016). Read more (PubMed: 26842342) »
See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your enquiry, we have received the following information from the source of those antibodies: Monoclonal antibodies ab9821 and 9822 were raised against the recombinant human growth hormone (hGH) cloned from mRNA (GenBank ac. No NM-000515) and expressed in E.coli. The cDNA sequence used for cloning is corresponding to the transcript variant hGH-N (protein isoform with MW 22kDa). The mature protein sequence (without signal sequence of 27 N-terminal amino acids) of hGH have been used for antibody production. Monoclonal antibodies ab9821 and ab9822 recognize full length hGH, both recombinant and natural. The reactivity of antibodies with different isoforms of hGH was however not tested. We hope this information helps, please do not hesitate to contact us if you need further advice,

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Answer

Binding capacity in serum was not determined for ab9821.

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Answer

You get 100 micro-litres for £195.00 sterling.

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