Key features and details
- Mouse monoclonal [GH-2] to Growth Hormone
- Suitable for: WB, ELISA, Indirect ELISA
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Growth Hormone antibody [GH-2]
See all Growth Hormone primary antibodies
DescriptionMouse monoclonal [GH-2] to Growth Hormone
Tested applicationsSuitable for: WB, ELISA, Indirect ELISAmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human Growth Hormone.
Database link: P01241
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR172544-1 and GR172544-3 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Concentration information loading...
Light chain typeunknown
Our Abpromise guarantee covers the use of ab9822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000.|
|ELISA||Use at an assay dependent dilution.
This antibody can be used in two-site immunoassays for the determination of human growth hormone levels. It recognizes a different epitope than GH-1 (ab9821). We recommend using this antibody (ab9822) as the detection antibody
|Indirect ELISA||1/1000 - 1/10000.|
FunctionPlays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
Involvement in diseaseDefects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy.
Sequence similaritiesBelongs to the somatotropin/prolactin family.
- Information by UniProt
- gH antibody
- GH-N antibody
- GH1 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab9822 has been referenced in 3 publications.
- Fradkin AH et al. Immunogenicity of aggregates of recombinant human growth hormone in mouse models. J Pharm Sci 98:3247-64 (2009). ELISA ; Mouse . PubMed: 19569057
- Kunert R et al. CHO-recombinant human growth hormone as a protease sensitive reporter protein. Appl Microbiol Biotechnol 84:693-9 (2009). PubMed: 19396439
- Markaki M et al. Stable expression of human growth hormone over 50 generations in transgenic insect larvae. Transgenic Res 16:99-107 (2007). PubMed: 17103025