Recombinant Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5291(2)] to Growth hormone receptor
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Growth hormone receptor antibody [EPR5291(2)]
See all Growth hormone receptor primary antibodies -
Description
Rabbit monoclonal [EPR5291(2)] to Growth hormone receptor -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Growth hormone receptor aa 550-650 (intracellular). The exact sequence is proprietary.
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Positive control
- WB: MCF-7, BxPC-3, and human fetal muscle, mouse brain and rat brain lysate. ICC/IF: HepG2 cells. Flow Cyt: MCF-7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 7.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5291(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
- MCF7 whole cell lysate (ab29537)
- MCF7 membrane extract lysate (ab29539)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 72 kDa).
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Flow Cyt |
1/420.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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ICC/IF | (1) |
1/200.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 72 kDa). |
Flow Cyt
1/420. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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ICC/IF
1/200. |
Target
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Function
Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway.
The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.
Isoform 2 up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling. -
Tissue specificity
Expressed in various tissues with high expression in liver and skeletal muscle. Isoform 4 is predominantly expressed in kidney, bladder, adrenal gland and brain stem. Isoform 1 expression in placenta is predominant in chorion and decidua. Isoform 4 is highly expressed in placental villi. Isoform 2 is expressed in lung, stomach and muscle. Low levels in liver. -
Involvement in disease
Defects in GHR are a cause of Laron syndrome (LARS) [MIM:262500]. A severe form of growth hormone insensitivity characterized by growth impairment, short stature, dysfunctional growth hormone receptor, and failure to generate insulin-like growth factor I in response to growth hormone.
Defects in GHR may be a cause of idiopathic short stature autosomal (ISSA) [MIM:604271]. Short stature is defined by a subnormal rate of growth. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 1 subfamily.
Contains 1 fibronectin type-III domain. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
The extracellular domain is the ligand-binding domain representing the growth hormone-binding protein (GHBP).
The ubiquitination-dependent endocytosis motif (UbE) is required for recruitment of the ubiquitin conjugation system on to the receptor and for its internalization. -
Post-translational
modificationsThe soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. Shedding is inhibited by growth hormone (GH) binding to the receptor probably due to a conformational change in GHR rendering the receptor inaccessible to ADAM17.
On GH binding, phosphorylated on tyrosine residues in the cytoplasmic domain by JAK2.
On ligand binding, ubiquitinated on lysine residues in the cytoplasmic domain. This ubiquitination is not sufficient for GHR internalization. -
Cellular localization
Secreted; Cell membrane. On growth hormone binding, GHR is ubiquitinated, internalized, down-regulated and transported into a degradative or non-degradative pathway and Cell membrane. Remains fixed to the cell membrane and is not internalized. - Information by UniProt
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Database links
- Entrez Gene: 2690 Human
- Entrez Gene: 14600 Mouse
- Entrez Gene: 25235 Rat
- Omim: 600946 Human
- SwissProt: P10912 Human
- SwissProt: P16882 Mouse
- SwissProt: P16310 Rat
- Unigene: 125180 Human
see all -
Alternative names
- GH receptor antibody
- GH-binding protein antibody
- GHBP antibody
see all
Images
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Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078) at 1/2000 dilution (purified) + BxPC-3 cell lysate at 10 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 72 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078) at 1/10000 dilution (purified) + MCF-7 cell lysate at 10 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 72 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078) at 1/10000 dilution (purified)
Lane 1 : Mouse brain
Lane 2 : Rat brain
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 72 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Immunocytochemistry/ Immunofluorescence - Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078)
Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling Growth hormone receptor with purified ab134078 at 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Control: primary antibody (1/500) and secondary antibody ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/400).
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Immunocytochemistry/ Immunofluorescence - Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078)This image is courtesy of an anonymous Abreview
ab134078 (unpurified) staining Growth hormone receptor in a human glioblastoma cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde,permeabilized with TBS Tween 0.5% and blocked for 60 minutes at 25°C. Samples were incubated with primary antibody (1/100) for 20 hour at 4°C. An Alexa Fluor® 594-conjugated Goat anti-rabbit IgG polyclonal(1/200) was used as the secondary antibody.
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Flow cytometry analysis of MCF-7 cells labelling Growth hormone receptor with purified ab134078 at 1/420 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab134078 has been referenced in 5 publications.
- Nemoto T & Kakinuma Y Fetal malnutrition-induced catch up failure is caused by elevated levels of miR-322 in rats. Sci Rep 10:1339 (2020). PubMed: 31992823
- Mao J et al. An improved method of culturing somatotropic cells from rat adenohypophysis. Tissue Cell 58:93-98 (2019). PubMed: 31133252
- Basu R et al. Targeting growth hormone receptor in human melanoma cells attenuates tumor progression and epithelial mesenchymal transition via suppression of multiple oncogenic pathways. Oncotarget 8:21579-21598 (2017). WB, ICC/IF ; Human . PubMed: 28223541
- Lev A et al. Preclinical rationale for combination of crizotinib with mitomycin C for the treatment of advanced colorectal cancer. Cancer Biol Ther 18:694-704 (2017). PubMed: 28886275
- Lee EH et al. Immunogenomics reveal molecular circuits of diclofenac induced liver injury in mice. Oncotarget 7:14983-5017 (2016). PubMed: 26934552