Anti-GRP antibody (ab130401)
Key features and details
- Rabbit polyclonal to GRP
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GRP antibody -
Description
Rabbit polyclonal to GRP -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment: LNNSLSSPVQ ADVYFPRLIV PFCGHIKGGM RPGKKVLVMG IVDLNPESFA ISLTCGDSED PPADVAIELK, corresponding to amino acids 19-88 of Human GRP (Q3ZCW2).
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Positive control
- Human liver tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab130401 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Does not bind lactose, and may not bind carbohydrates. -
Sequence similarities
Contains 1 galectin domain. - Information by UniProt
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Database links
- Entrez Gene: 29094 Human
- SwissProt: Q3ZCW2 Human
- Unigene: 372208 Human
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Alternative names
- Galectin-related protein antibody
- GRP antibody
- HSPC159 antibody
see all
Images
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Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab130401 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemical analysis of Formalin-fixed, paraffin embedded human liver tissue labeled with GRP antibody at 1/10 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab130401 has been referenced in 1 publication.
- Meng L et al. Ultrasound-microbubbles-mediated microRNA-449a inhibits lung cancer cell growth via the regulation of Notch1. Onco Targets Ther 12:7437-7450 (2019). PubMed: 31686849