Anti-GRP antibody (ab130401)

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Overview

  • Product name

    Anti-GRP antibody

  • Description

    Rabbit polyclonal to GRP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment: LNNSLSSPVQ ADVYFPRLIV PFCGHIKGGM RPGKKVLVMG IVDLNPESFA ISLTCGDSED PPADVAIELK, corresponding to amino acids 19-88 of Human GRP (Q3ZCW2).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human liver tissue.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab130401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Does not bind lactose, and may not bind carbohydrates.

  • Sequence similarities

    Contains 1 galectin domain.
  • Information by UniProt

  • Database links

    • Alternative names

      • Galectin-related protein antibody
      • GRP antibody
      • HSPC159 antibody
      • lectin, galactoside-binding-like antibody
      • LEGL_HUMAN antibody
      • LGALSL antibody
      • MGC188016 antibody
      • MGC33751 antibody
      • MGC71953 antibody
      • RGD1307414 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-GRP antibody (ab130401)
      Immunocytochemistry/ Immunofluorescence - Anti-GRP antibody (ab130401)
      Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab130401 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP antibody (ab130401)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP antibody (ab130401)

      Immunohistochemical analysis of Formalin-fixed, paraffin embedded human liver tissue labeled with GRP antibody at 1/10 dilution.

    References

    ab130401 has not yet been referenced specifically in any publications.

    Publishing research using ab130401? Please let us know so that we can cite the reference in this datasheet.

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