Product nameAnti-Grp75 / MOT antibody [9F8]
See all Grp75 / MOT primary antibodies
DescriptionMouse monoclonal [9F8] to Grp75 / MOT
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Rat, Human, Non human primates
Recombinant full length protein corresponding to Human Grp75/ MOT. Expressed in 293T cells.
Database link: Q8N1C8
- 293T, HeLa, K562, A431, COS7 and NRK lysates; HeLa cells.
Previously labelled as Grp75.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 30% Glycerol, 69% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab171089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 74 kDa.|
|IP||Use at an assay dependent concentration.
FunctionImplicated in the control of cell proliferation and cellular aging. May also act as a chaperone.
Sequence similaritiesBelongs to the heat shock protein 70 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 75 kDa glucose regulated protein antibody
- 75 kDa glucose-regulated protein antibody
- CSA antibody
All lanes : Anti-Grp75 / MOT antibody [9F8] (ab171089) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : A431 whole cell lysate
Lane 5 : COS7 whole cell lysate
Lane 6 : NIH3T3 whole cell lysate
Lane 7 : NRK whole cell lysate
Lysates/proteins at 50 µg per lane.
All lanes : Goat anti-Mouse HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 74 kDa
4-20% Tris-HCL polyacrylamide gel
Immunofluorescent analysis of HeLa cells labeling Grp75 / MOT with ab171089 at 1/50 dilution (green) compared to negative control NIH3T3 cells.
Detection of Grp75 / MOT in Immunoprecipitates of HeLa whole cell lysates using ab171089 at 2µg per 750µg lysate. Detected with ab171089 at 1/1000 dilution.
This product has been referenced in:
- Xie G et al. An investigation of methyl tert-butyl ether-induced cytotoxicity and protein profile in Chinese hamster ovary cells. Mol Med Rep 16:8595-8604 (2017). Read more (PubMed: 29039499) »
- Martínez-Pizarro A et al. Endoplasmic Reticulum Stress and Autophagy in Homocystinuria Patients with Remethylation Defects. PLoS One 11:e0150357 (2016). WB . Read more (PubMed: 26959487) »