Overview

  • Product name
    Anti-Grp75 antibody [9F8]
    See all Grp75 primary antibodies
  • Description
    Mouse monoclonal [9F8] to Grp75
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Rat, Human, Non human primates
  • Immunogen

    Recombinant full length protein corresponding to Human Grp75. Expressed in 293T cells.
    Database link: Q8N1C8

  • Positive control
    • 293T, HeLa, K562, A431, COS7 and NRK lysates; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab171089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 74 kDa.
ICC/IF 1/50.
IP Use at an assay dependent concentration.

Use 2µg.

Target

  • Function
    Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone.
  • Sequence similarities
    Belongs to the heat shock protein 70 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 75 kDa glucose regulated protein antibody
    • 75 kDa glucose-regulated protein antibody
    • CSA antibody
    • Glucose Regulated Protein antibody
    • Grp 75 antibody
    • GRP-75 antibody
    • GRP75 antibody
    • GRP75_HUMAN antibody
    • Heat shock 70 kDa protein 9 antibody
    • Heat shock 70kD protein 9 antibody
    • heat shock 70kDa protein 9 antibody
    • Heat shock 70kDa protein 9B antibody
    • Heat shock protein 74 kDa A antibody
    • Heat shock protein A antibody
    • Heat shock protein cognate 74 antibody
    • Hsc74 antibody
    • Hsp74 antibody
    • Hsp74a antibody
    • HSPA9 antibody
    • Hspa9a antibody
    • HSPA9B antibody
    • MGC4500 antibody
    • mitochondrial antibody
    • Mortalin 2 antibody
    • Mortalin antibody
    • Mortalin perinuclear antibody
    • Mortalin2 antibody
    • MOT 2 antibody
    • MOT antibody
    • MOT2 antibody
    • Mthsp70 antibody
    • p66 mortalin antibody
    • P66 MOT antibody
    • PBP74 antibody
    • Peptide binding protein 74 antibody
    • Peptide-binding protein 74 antibody
    • Stress 70 protein mitochondrial antibody
    • Stress 70 protein mitochondrial precursor antibody
    • Stress-70 protein antibody
    see all

Images

  • All lanes : Anti-Grp75 antibody [9F8] (ab171089) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : K562 whole cell lysate
    Lane 4 : A431 whole cell lysate
    Lane 5 : COS7 whole cell lysate
    Lane 6 : NIH3T3 whole cell lysate
    Lane 7 : NRK whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse HRP at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 74 kDa



    4-20% Tris-HCL polyacrylamide gel

  • Immunofluorescent analysis of HeLa cells labeling Grp75 with ab171089 at 1/50 dilution (green) compared to negative control NIH3T3 cells. 

  • Detection of Grp75 in Immunoprecipitates of HeLa whole cell lysates using ab171089 at 2µg per 750µg lysate. Detected with ab171089 at 1/1000 dilution.

References

This product has been referenced in:
  • Martínez-Pizarro A  et al. Endoplasmic Reticulum Stress and Autophagy in Homocystinuria Patients with Remethylation Defects. PLoS One 11:e0150357 (2016). WB . Read more (PubMed: 26959487) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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