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Mouse monoclonal [9F8] to Grp75
Rat, Human, Non human primates
Recombinant full length protein corresponding to Human Grp75. Expressed in 293T cells.
Database link: Q8N1C8
- 293T, HeLa, K562, A431, COS7 and NRK lysates; HeLa cells.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 30% Glycerol, 69% PBS
Concentration information loading...
Protein A purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/1000. Predicted molecular weight: 74 kDa.
||Use at an assay dependent concentration.
Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone.
Belongs to the heat shock protein 70 family.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
- 75 kDa glucose regulated protein antibody
- 75 kDa glucose-regulated protein antibody
- CSA antibody
Western blot - Anti-Grp75 antibody [9F8] (ab171089)
All lanes :
Anti-Grp75 antibody [9F8] (ab171089) at 1/1000 dilutionLane 1 :
293T whole cell lysateLane 2 :
HeLa whole cell lysateLane 3 :
K562 whole cell lysateLane 4 :
A431 whole cell lysateLane 5 :
COS7 whole cell lysateLane 6 :
NIH3T3 whole cell lysateLane 7 :
NRK whole cell lysate
Lysates/proteins at 50 µg per lane.SecondaryAll lanes :
Goat anti-Mouse HRP at 1/20000 dilution
Developed using the ECL technique.Predicted band size:
4-20% Tris-HCL polyacrylamide gel
Immunocytochemistry/ Immunofluorescence - Anti-Grp75 antibody [9F8] (ab171089)
Immunofluorescent analysis of HeLa cells labeling Grp75 with ab171089 at 1/50 dilution (green) compared to negative control NIH3T3 cells.
Immunoprecipitation - Anti-Grp75 antibody [9F8] (ab171089)
Detection of Grp75 in Immunoprecipitates of HeLa whole cell lysates using ab171089 at 2µg per 750µg lysate. Detected with ab171089 at 1/1000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"