The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 for 30 minutes. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
WB: Use at an assay dependent dilution. Predicted molecular weight: 75 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone.
Belongs to the heat shock protein 70 family.
Phosphorylated upon DNA damage, probably by ATM or ATR.