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Our Abpromise guarantee covers the use of ab2799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).|
|IP||Use at an assay dependent concentration.|
|ICC/IF||1/50 - 1/200.
Staining of mtHSP 70 in DAP.3 cells results in a worm-like staining pattern, consistent with mitochondrial localization.
|IHC-P||Use a concentration of 1 µg/ml.|
ab91537 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.
Immunocytochemistry/Immunofluorescence analysis of Grp75 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA for 15 minutes at room temperature. Cells were incubated with (left panel) or without (right panel) ab2799 (1:50) for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody (1:400) for 30 minutes at room temperature. F-Actin (red) was stained with Dylight 554 phalloidin, and nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.
Primary incubated for 1 hour 30 minutes at 24°C.
Immunocytochemistry/Immunofluorescence analysis of human fibroblasts labelling Grp75 with ab2799.
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