Anti-Grp75/MOT antibody (ab124382)
Key features and details
- Rabbit polyclonal to Grp75/MOT
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-Grp75/MOT antibody
See all Grp75/MOT primary antibodies -
Description
Rabbit polyclonal to Grp75/MOT -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human Grp75/MOT (C terminal).
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General notes
Previously labelled as Grp75.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS without Mg2+, Ca2+ -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124382 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 74 kDa. | |
| IHC-P | Use a concentration of 5 µg/ml. | |
| ICC/IF | 1/100 - 1/500. |
Target
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Function
Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone. -
Sequence similarities
Belongs to the heat shock protein 70 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 3313 Human
- Entrez Gene: 15526 Mouse
- Entrez Gene: 291671 Rat
- Omim: 600548 Human
- SwissProt: P38646 Human
- SwissProt: P38647 Mouse
- SwissProt: P48721 Rat
- Unigene: 184233 Human
see all -
Alternative names
- 75 kDa glucose regulated protein antibody
- 75 kDa glucose-regulated protein antibody
- CSA antibody
see all
Images
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Western blot - Anti-Grp75/MOT antibody (ab124382)All lanes : Anti-Grp75/MOT antibody (ab124382) at 1/500 dilution
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
Lane 2 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract with synthesized peptide
Predicted band size: 74 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grp75/MOT antibody (ab124382)ab124382, at 5 µg/ml, staining Grp75/MOT in formalin fixed, paraffin embedded Human prostate by Immunohistochemistry.
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Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody (ab124382)ab124382, at 1/100, staining Grp75/MOT in COS-7 (African green monkey kidney fibroblast-like cell line) cells by Immunofluorescence. The image on the right has ben treated with the synthesized peptide.
Protocols
Datasheets and documents
References (0)
ab124382 has not yet been referenced specifically in any publications.
