Anti-Grp75/MOT antibody (ab124382)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Grp75/MOT antibody
See all Grp75/MOT primary antibodies -
Description
Rabbit polyclonal to Grp75/MOT -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISA, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human Grp75/MOT (C terminal).
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Positive control
- Human prostate tissue; COS7 cell extract; COS7 cells.
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General notes
Previously labelled as Grp75.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 0.88% Sodium chloride
Note: PBS without Mg2+, Ca2+ -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124382 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 74 kDa. | |
| ELISA | 1/5000. | |
| IHC-P | Use a concentration of 5 µg/ml. | |
| ICC/IF | 1/100 - 1/500. |
Target
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Function
Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone. -
Sequence similarities
Belongs to the heat shock protein 70 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 3313 Human
- Entrez Gene: 15526 Mouse
- Entrez Gene: 291671 Rat
- Omim: 600548 Human
- SwissProt: P38646 Human
- SwissProt: P38647 Mouse
- SwissProt: P48721 Rat
- Unigene: 184233 Human
see all -
Alternative names
- 75 kDa glucose regulated protein antibody
- 75 kDa glucose-regulated protein antibody
- CSA antibody
see all
Images
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Western blot - Anti-Grp75/MOT antibody (ab124382)All lanes : Anti-Grp75/MOT antibody (ab124382) at 1/500 dilution
Lane 1 : COS7 cell extract
Lane 2 : COS7 cell extract with synthesized peptide
Predicted band size: 74 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grp75/MOT antibody (ab124382)ab124382, at 5 µg/ml, staining Grp75/MOT in formalin fixed, paraffin embedded Human prostate by Immunohistochemistry.
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Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody (ab124382)ab124382, at 1/100, staining Grp75/MOT in COS7 cells by Immunofluorescence. The image on the right has ben treated with the synthesized peptide.
Protocols
Datasheets and documents
References
ab124382 has not yet been referenced specifically in any publications.
