Key features and details
- Rabbit polyclonal to Grp75/MOT
- Suitable for: IP, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Grp75/MOT antibody
See all Grp75/MOT primary antibodies
DescriptionRabbit polyclonal to Grp75/MOT
Tested applicationsSuitable for: IP, IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Xenopus laevis
Recombinant fragment within Human Grp75/MOT (internal sequence). The exact sequence is proprietary.
Database link: P38646
- WB: HEK-293T, A431, HeLa, HepG2 and SCC-15 whole cell extract. IHC-P: Mouse duodenum tissue; rat duodenum and brain tissues. IP: HepG2 whole cell extract.
Previously labelled as Grp75.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/100 - 1/500.|
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 74 kDa.|
FunctionImplicated in the control of cell proliferation and cellular aging. May also act as a chaperone.
Sequence similaritiesBelongs to the heat shock protein 70 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 75 kDa glucose regulated protein antibody
- 75 kDa glucose-regulated protein antibody
- CSA antibody
All lanes : Anti-Grp75/MOT antibody (ab227215) at 1/2500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 74 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded rat duodenum tissue stained for Grp75/MOT using ab227215 at 1/500 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence of HeLa cells staining Grp75/MOT with ab227215 (1/500). Hoechst 33342 staining. (blue).
Anti-Grp75/MOT antibody (ab227215) at 1/500 dilution + SCC-15 (Human squamous cell carcinoma, tongue) whole cell extract at 6 µg
Predicted band size: 74 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded mouse duodenum tissue stained for Grp75/MOT using ab227215 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded rat brain tissue stained for Grp75/MOT using ab227215 at 1/500 dilution in immunohistochemical analysis.
Grp75/MOT was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract with 5 µg of ab227215. Western blot was performed from the immunoprecipitate using ab227215. Anti-Rabbit IgG was used as secondary antibody.
Lane 1: Control IgG instead of ab227215 in HepG2 whole cell extract.
Lane 2: ab227215 IP in HepG2 whole cell extract.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227215 has not yet been referenced specifically in any publications.