Product nameAnti-Grp75/MOT antibody - C-terminal
See all Grp75/MOT primary antibodies
DescriptionRabbit polyclonal to Grp75/MOT - C-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human Grp75/MOT aa 629-679 (C terminal). The exact sequence is proprietary.
Database link: P38646
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Previously labelled as Grp75.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226379 was affinity purified using an epitope specific to Grp75/MOT immobilized on solid support.
Our Abpromise guarantee covers the use of ab226379 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 74 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionImplicated in the control of cell proliferation and cellular aging. May also act as a chaperone.
Sequence similaritiesBelongs to the heat shock protein 70 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 75 kDa glucose regulated protein antibody
- 75 kDa glucose-regulated protein antibody
- CSA antibody
All lanes : Anti-Grp75/MOT antibody - C-terminal (ab226379) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 74 kDa
Exposure time: 1 second
Lysates prepared using NETN lysis buffer.
Grp75/MOT was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226379 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226379 at 1 µg/ml.
Lane 1: ab226379 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 1 second.
ab226379 has not yet been referenced specifically in any publications.