Product nameAnti-Grp75/MOT antibody [EPR5326(2)]
See all Grp75/MOT primary antibodies
DescriptionRabbit monoclonal [EPR5326(2)] to Grp75/MOT
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Grp75/MOT aa 650-750 (C terminal). The exact sequence is proprietary.
- HeLa, Jurkat, K562, and MCF-7 cell lysates
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 4.30 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 74 kDa).|
FunctionImplicated in the control of cell proliferation and cellular aging. May also act as a chaperone.
Sequence similaritiesBelongs to the heat shock protein 70 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 75 kDa glucose regulated protein antibody
- 75 kDa glucose-regulated protein antibody
- CSA antibody
All lanes : Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lane 4 : MCF-7 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab129201 has not yet been referenced specifically in any publications.