Recombinant Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201)
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- References
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Overview
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Product name
Anti-Grp75/MOT antibody [EPR5326(2)]
See all Grp75/MOT primary antibodies -
Description
Rabbit monoclonal [EPR5326(2)] to Grp75/MOT -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Grp75/MOT aa 650-750 (C terminal). The exact sequence is proprietary.
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Positive control
- HeLa, Jurkat, K562, and MCF-7 cell lysates
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General notes
Previously labelled as Grp75.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5326(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab129201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 74 kDa). |
Target
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Function
Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone. -
Sequence similarities
Belongs to the heat shock protein 70 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 3313 Human
- Entrez Gene: 15526 Mouse
- Entrez Gene: 291671 Rat
- Omim: 600548 Human
- SwissProt: P38646 Human
- SwissProt: P38647 Mouse
- SwissProt: P48721 Rat
- Unigene: 184233 Human
see all -
Alternative names
- 75 kDa glucose regulated protein antibody
- 75 kDa glucose-regulated protein antibody
- CSA antibody
see all
Images
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![Western blot - Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201)](https://www.abcam.com/ps/products/129/ab129201/Images/ab129201-143260-anti-grp75-mot-antibody-epr53262-western-blot.jpg)
Western blot - Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201)All lanes : Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lane 4 : MCF-7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
![Other - Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201)](https://www.abcam.com/ps/products/129/ab129201/Images/ab129201-143259-anti-grp75-mot-antibody-epr53262.jpg)
Other - Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201)
Protocols
Datasheets and documents
References
ab129201 has not yet been referenced specifically in any publications.
