Recombinant Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201)
![Western blot - Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201)](https://www.abcam.com/ps/products/129/ab129201/Images/ab129201-143260-anti-grp75-mot-antibody-epr53262-western-blot.jpg "Western blot - Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5326(2)] to Grp75/MOT
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Grp75/MOT antibody [EPR5326(2)]
See all Grp75/MOT primary antibodies -
Description
Rabbit monoclonal [EPR5326(2)] to Grp75/MOT -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Grp75/MOT aa 650-750 (C terminal). The exact sequence is proprietary.
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Positive control
- HeLa, Jurkat, K562, and MCF-7 cell lysates
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General notes
Previously labelled as Grp75.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5326(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab129201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 74 kDa). |
Target
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Function
Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone. -
Sequence similarities
Belongs to the heat shock protein 70 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 3313 Human
- Entrez Gene: 15526 Mouse
- Entrez Gene: 291671 Rat
- Omim: 600548 Human
- SwissProt: P38646 Human
- SwissProt: P38647 Mouse
- SwissProt: P48721 Rat
- Unigene: 184233 Human
see all -
Alternative names
- 75 kDa glucose regulated protein antibody
- 75 kDa glucose-regulated protein antibody
- CSA antibody
see all
Images
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Western blot - Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201)All lanes : Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lane 4 : MCF-7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
![OI-RD Scanning - Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201)](https://www.abcam.com/ps/products/129/ab129201/Images/ab129201-143259-anti-grp75-mot-antibody-epr53262.jpg)
OI-RD Scanning - Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201) -
![Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201)](https://www.abcam.com/ps/products/129/ab129201/Images/ab129201-3-benefits-of-recombinant-antibodies.png)
Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201)
Datasheets and documents
References (1)
ab129201 has been referenced in 1 publication.
- Nadeem A et al. Beta-sheet-specific interactions with heat shock proteins define a mechanism of delayed tumor cell death in response to HAMLET. J Mol Biol 431:2612-2627 (2019). PubMed: 31082436
