Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5326(2)] to Grp75/MOT - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-Grp75/MOT antibody [EPR5326(2)] - BSA and Azide free
See all Grp75/MOT primary antibodies
DescriptionRabbit monoclonal [EPR5326(2)] to Grp75/MOT - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Grp75/MOT aa 650-750 (C terminal). The exact sequence is proprietary.
Ab248348 is the carrier-free version of ab129201. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248348 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Dissociation constant (KD)KD = 4.30 x 10 -11 M Learn more about KD
Storage bufferpH: 7.2
Concentration information loading...
Our Abpromise guarantee covers the use of ab248348 in the following tested applications.
|WB||Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 74 kDa).|
FunctionImplicated in the control of cell proliferation and cellular aging. May also act as a chaperone.
Sequence similaritiesBelongs to the heat shock protein 70 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 75 kDa glucose regulated protein antibody
- 75 kDa glucose-regulated protein antibody
- CSA antibody
All lanes : Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lane 4 : MCF-7 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
This data was developed using ab129201, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab248348 has not yet been referenced specifically in any publications.