Overview

  • Product name

    Anti-Grp75/MOT antibody [N52A/42]
    See all Grp75/MOT primary antibodies
  • Description

    Mouse monoclonal [N52A/42] to Grp75/MOT
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse Grp75/MOT aa 551-766.

  • Positive control

    • WB: HeLa cell lysate. ICC/IF Heat shocked HeLa cells.
  • General notes

    The clone number has been updated from S52A-42 to N52A/42, both clone numbers name the same antibody clone.

     

    Previously labelled as Grp75. 

Properties

Applications

Our Abpromise guarantee covers the use of ab94668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB 1/1000. Predicted molecular weight: 74 kDa.

Target

  • Function

    Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone.
  • Sequence similarities

    Belongs to the heat shock protein 70 family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • 75 kDa glucose regulated protein antibody
    • 75 kDa glucose-regulated protein antibody
    • CSA antibody
    • Glucose Regulated Protein antibody
    • Grp 75 antibody
    • GRP-75 antibody
    • GRP75 antibody
    • GRP75_HUMAN antibody
    • Heat shock 70 kDa protein 9 antibody
    • Heat shock 70kD protein 9 antibody
    • heat shock 70kDa protein 9 antibody
    • Heat shock 70kDa protein 9B antibody
    • Heat shock protein 74 kDa A antibody
    • Heat shock protein A antibody
    • Heat shock protein cognate 74 antibody
    • Hsc74 antibody
    • Hsp74 antibody
    • Hsp74a antibody
    • HSPA9 antibody
    • Hspa9a antibody
    • HSPA9B antibody
    • MGC4500 antibody
    • mitochondrial antibody
    • Mortalin 2 antibody
    • Mortalin antibody
    • Mortalin perinuclear antibody
    • Mortalin2 antibody
    • MOT 2 antibody
    • MOT antibody
    • MOT2 antibody
    • Mthsp70 antibody
    • p66 mortalin antibody
    • P66 MOT antibody
    • PBP74 antibody
    • Peptide binding protein 74 antibody
    • Peptide-binding protein 74 antibody
    • Stress 70 protein mitochondrial antibody
    • Stress 70 protein mitochondrial precursor antibody
    • Stress-70 protein antibody
    see all

Images

  • Anti-Grp75/MOT antibody [N52A/42] (ab94668) at 1/1000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg

    Secondary
    Sheep Anti-Mouse IgG: HRP

    Predicted band size: 74 kDa



    Block: 1.5% BSA for 30 minutes at RT.

    Incubated with primary antibody for 2 hours at RT.

    Incubated with secondary antibody for 1 hour at RT.

  • HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Grp75/MOT (Green) using ab94668 at a 1/100 dilution in ICC/IF.

    Heat shocked HeLa cells were fixed in 2% formaldehyde for 20 minutes at RT. Incubated with primary antibody for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40,000 for 2 hours at RT.

References

ab94668 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

Thank you for calling Abcam earlier.
I have talked to the lab about the immunogen for ab94668 and they have told me that, this antibody was raised against SALM2, not Grp75. The outcome was an excellent antibody to Grp75 - I don't have a terribly good answer as to why that might be... But that's the way it turned out, living system sometimes do interesting things.
In regards to your enquiry, this antibody does recognize the C-terminal end of the protein and so would not be a good choice for your IPs.
If there is anything else I can help you with, please let me know.

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