Product nameAnti-Grp75/MOT antibody [N52A/42]
See all Grp75/MOT primary antibodies
DescriptionMouse monoclonal [N52A/42] to Grp75/MOT
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Mouse Grp75/MOT aa 551-766.
- WB: HeLa cell lysate. ICC/IF Heat shocked HeLa cells.
The clone number has been updated from S52A-42 to N52A/42, both clone numbers name the same antibody clone.
Previously labelled as Grp75.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab94668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 74 kDa.|
FunctionImplicated in the control of cell proliferation and cellular aging. May also act as a chaperone.
Sequence similaritiesBelongs to the heat shock protein 70 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 75 kDa glucose regulated protein antibody
- 75 kDa glucose-regulated protein antibody
- CSA antibody
Anti-Grp75/MOT antibody [N52A/42] (ab94668) at 1/1000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg
Sheep Anti-Mouse IgG: HRP
Predicted band size: 74 kDa
Block: 1.5% BSA for 30 minutes at RT.
Incubated with primary antibody for 2 hours at RT.
Incubated with secondary antibody for 1 hour at RT.
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Grp75/MOT (Green) using ab94668 at a 1/100 dilution in ICC/IF.
Heat shocked HeLa cells were fixed in 2% formaldehyde for 20 minutes at RT. Incubated with primary antibody for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40,000 for 2 hours at RT.
ab94668 has not yet been referenced specifically in any publications.