Overview

  • Product name

    Anti-GRP78 BiP antibody [3C5-1A4]
    See all GRP78 BiP primary antibodies
  • Description

    Mouse monoclonal [3C5-1A4] to GRP78 BiP
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Rat GRP78 BiP.
    Database link: P06761

  • Positive control

    • ICC/IF: NIH/3T3 cells. WB: NIH/3T3 and HEK-293 cell lysate; Rat brain lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    3C5-1A4
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab212054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 72 kDa.
ICC/IF 1/100.

Target

  • Function

    Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
  • Involvement in disease

    Autoantigen in rheumatoid arthritis.
  • Sequence similarities

    Belongs to the heat shock protein 70 family.
  • Cellular localization

    Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • 78 kDa glucose regulated protein antibody
    • 78 kDa glucose-regulated protein antibody
    • AL022860 antibody
    • AU019543 antibody
    • BIP antibody
    • D2Wsu141e antibody
    • D2Wsu17e antibody
    • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody
    • Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody
    • Epididymis secretory sperm binding protein Li 89n antibody
    • FLJ26106 antibody
    • Glucose Regulated Protein 78 antibody
    • Glucose Regulated Protein 78kDa antibody
    • GRP 78 antibody
    • GRP-78 antibody
    • GRP78 antibody
    • GRP78_HUMAN antibody
    • Heat shock 70 kDa protein 5 antibody
    • Heat Shock 70kDa Protein 5 antibody
    • Heat shock protein family A (Hsp70) member 5 antibody
    • HEL S 89n antibody
    • Hsce70 antibody
    • HSPA 5 antibody
    • HSPA5 antibody
    • Immunoglobulin Heavy Chain Binding Protein antibody
    • Immunoglobulin heavy chain-binding protein antibody
    • mBiP antibody
    • MIF2 antibody
    • Sez7 antibody
    see all

Images

  • 4% formaldehyde-fixed NIH/3T3 (mouse embyro fibroblast cell line) cells stained for GRP78 BiP (green) using ab212054 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Mouse 488 at 1/100 for 60 minutes at RT. Counterstain: DAPI (blue) nuclear stain at 1/5000 for 5 minutes RT.

    (A) DAPI nuclear stain (B) ab212054 (C) Merge.

  • All lanes : Anti-GRP78 BiP antibody [3C5-1A4] (ab212054) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
    Lane 2 : Rat brain lysate
    Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

    Secondary
    All lanes : HRP Goat Anti-Mouse at 1/50 dilution

    Predicted band size: 72 kDa



    Blocking: 5% milk + TBST for 1 hour at RT.

    Color Development: TMB solution for 5 minutes at RT.

References

ab212054 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab212054.
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