Key features and details
- Mouse monoclonal [3C5-1A4] to GRP78 BiP
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-GRP78 BiP antibody [3C5-1A4]
See all GRP78 BiP primary antibodies
DescriptionMouse monoclonal [3C5-1A4] to GRP78 BiP
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Rat GRP78 BiP.
Database link: P06761
- ICC/IF: NIH/3T3 cells. WB: NIH/3T3 and HEK-293 cell lysate; Rat brain lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab212054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 72 kDa.|
FunctionProbably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
Involvement in diseaseAutoantigen in rheumatoid arthritis.
Sequence similaritiesBelongs to the heat shock protein 70 family.
Cellular localizationEndoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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4% formaldehyde-fixed NIH/3T3 (mouse embyro fibroblast cell line) cells stained for GRP78 BiP (green) using ab212054 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Mouse 488 at 1/100 for 60 minutes at RT. Counterstain: DAPI (blue) nuclear stain at 1/5000 for 5 minutes RT.
(A) DAPI nuclear stain (B) ab212054 (C) Merge.
All lanes : Anti-GRP78 BiP antibody [3C5-1A4] (ab212054) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2 : Rat brain lysate
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
All lanes : HRP Goat Anti-Mouse at 1/50 dilution
Predicted band size: 72 kDa
Blocking: 5% milk + TBST for 1 hour at RT.
Color Development: TMB solution for 5 minutes at RT.
ab212054 has not yet been referenced specifically in any publications.