Anti-GRP78 BiP antibody (ab109659)
Key features and details
- Rabbit polyclonal to GRP78 BiP
- Suitable for: WB
- Reacts with: Mouse, Dog, Human
- Isotype: IgG
Overview
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Product name
Anti-GRP78 BiP antibody
See all GRP78 BiP primary antibodies -
Description
Rabbit polyclonal to GRP78 BiP -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Dog, Human -
Immunogen
Recombinant full length Human GRP78 BiP (His tagged at the N terminus).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Detects a band of approximately 78 kDa (predicted molecular weight: 72 kDa).
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Notes |
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WB
1/1000 - 1/2000. Detects a band of approximately 78 kDa (predicted molecular weight: 72 kDa). |
Target
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Function
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. -
Involvement in disease
Autoantigen in rheumatoid arthritis. -
Sequence similarities
Belongs to the heat shock protein 70 family. -
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 480726 Dog
- Entrez Gene: 3309 Human
- Entrez Gene: 14828 Mouse
- Omim: 138120 Human
- SwissProt: P11021 Human
- SwissProt: P20029 Mouse
- Unigene: 743241 Human
- Unigene: 330160 Mouse
see all -
Alternative names
- 78 kDa glucose regulated protein antibody
- 78 kDa glucose-regulated protein antibody
- AL022860 antibody
see all
Images
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All lanes : Anti-GRP78 BiP antibody (ab109659) at 1 µg/ml
Lane 1 : Recombinant Human GRP78 BiP at 0.1 µg
Lane 2 : SKBR3 lysate (Human) at 7.5 µg
Lane 3 : MDCK lysate (Dog) at 7.5 µg
Lane 4 : MEF lysate (Mouse) at 7.5 µg
Predicted band size: 72 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab109659 has been referenced in 1 publication.
- Wang Y et al. Blocking the interaction between interleukin-17A and endoplasmic reticulum stress in macrophage attenuates retinal neovascularization in oxygen-induced retinopathy. Cell Biosci 11:82 (2021). PubMed: 33933165