Key features and details
- Rabbit polyclonal to GRP78 BiP
- Suitable for: WB
- Reacts with: Mouse, Rat, Dog, Human
- Isotype: IgG
Product nameAnti-GRP78 BiP antibody
See all GRP78 BiP primary antibodies
DescriptionRabbit polyclonal to GRP78 BiP
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Dog, Human
Recombinant full length Human GRP78 BiP (His tagged at the N terminus).
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab109659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 78 kDa (predicted molecular weight: 72 kDa).|
FunctionProbably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
Involvement in diseaseAutoantigen in rheumatoid arthritis.
Sequence similaritiesBelongs to the heat shock protein 70 family.
Cellular localizationEndoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 78 kDa glucose regulated protein antibody
- 78 kDa glucose-regulated protein antibody
- AL022860 antibody
All lanes : Anti-GRP78 BiP antibody (ab109659) at 1 µg/ml
Lane 1 : Recombinant Human GRP78 BiP at 0.1 µg
Lane 2 : SKBR3 lysate (Human) at 7.5 µg
Lane 3 : MDCK lysate (Dog) at 7.5 µg
Lane 4 : MEF lysate (Mouse) at 7.5 µg
Predicted band size: 72 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
ab109659 has not yet been referenced specifically in any publications.