Overview

  • Product name

  • Description

    Rabbit polyclonal to GRP78 BiP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to Human GRP78.
    Database link: P11021

  • Positive control

    • Human brain cortex and testis tissue; Human prostate carcinoma tissue; NCI-H460 and HeLa cells; Mouse liver and HL60 cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Ammonium Sulphate Precipitation
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab188878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/10 - 1/50.
WB 1/1000. Predicted molecular weight: 72 kDa.

Target

  • Function

    Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
  • Involvement in disease

    Autoantigen in rheumatoid arthritis.
  • Sequence similarities

    Belongs to the heat shock protein 70 family.
  • Cellular localization

    Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • 78 kDa glucose regulated protein antibody
    • 78 kDa glucose-regulated protein antibody
    • AL022860 antibody
    • AU019543 antibody
    • BIP antibody
    • D2Wsu141e antibody
    • D2Wsu17e antibody
    • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody
    • Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody
    • Epididymis secretory sperm binding protein Li 89n antibody
    • FLJ26106 antibody
    • Glucose Regulated Protein 78 antibody
    • Glucose Regulated Protein 78kDa antibody
    • GRP 78 antibody
    • GRP-78 antibody
    • GRP78 antibody
    • GRP78_HUMAN antibody
    • Heat shock 70 kDa protein 5 antibody
    • Heat Shock 70kDa Protein 5 antibody
    • Heat shock protein family A (Hsp70) member 5 antibody
    • HEL S 89n antibody
    • Hsce70 antibody
    • HSPA 5 antibody
    • HSPA5 antibody
    • Immunoglobulin Heavy Chain Binding Protein antibody
    • Immunoglobulin heavy chain-binding protein antibody
    • mBiP antibody
    • MIF2 antibody
    • Sez7 antibody
    see all

Images

  • Anti-GRP78 BiP antibody (ab188878) at 1/1000 dilution + Mouse liver lysate

    Predicted band size: 72 kDa

  • Confocal immunofluorescent analysis of NCI H460 cells labeling GRP78 with ab188878 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

  • Anti-GRP78 BiP antibody (ab188878) at 1/1000 dilution + HL60 cell lysate at 35 µg

    Predicted band size: 72 kDa

  • Flow cytometric analysis of HeLa cells labeling GRP78 with ab188878 at 1/10 dilution (right histogram) compared with a negative control cell (left)  followed by FITC-conjugated goat-anti-rabbit secondary antibody.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue labeling GRP78 with ab188878 at 10 µg/ml followed by peroxidase-conjugated secondary antibody and DAB staining.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labeling GRP78 with ab188878 at 10 µg/ml followed by peroxidase-conjugated secondary antibody and AEC staining.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labeling GRP78 with ab188878 at 10 µg/ml followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cortex tissue labeling GRP78 with ab188878 at 10 µg/ml followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

ab188878 has not yet been referenced specifically in any publications.

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