Overview

  • Product name

  • Description

    Rabbit polyclonal to GRP78 BiP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Xenopus laevis, Zebrafish, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human GRP78 BiP (internal sequence). The exact sequence is proprietary.
    Database link: P11021

  • Positive control

    • WB: HepG2, HEK-293T, A431, HeLa, NIH/3T3, JC, BCL-1, PC-12 and Rat2 whole cell extracts. ICC/IF: A431 cells. IHC-P: Mouse and rat brain tissues

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 72 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
  • Involvement in disease

    Autoantigen in rheumatoid arthritis.
  • Sequence similarities

    Belongs to the heat shock protein 70 family.
  • Cellular localization

    Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • 78 kDa glucose regulated protein antibody
    • 78 kDa glucose-regulated protein antibody
    • AL022860 antibody
    • AU019543 antibody
    • BIP antibody
    • D2Wsu141e antibody
    • D2Wsu17e antibody
    • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody
    • Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody
    • Epididymis secretory sperm binding protein Li 89n antibody
    • FLJ26106 antibody
    • Glucose Regulated Protein 78 antibody
    • Glucose Regulated Protein 78kDa antibody
    • GRP 78 antibody
    • GRP-78 antibody
    • GRP78 antibody
    • GRP78_HUMAN antibody
    • Heat shock 70 kDa protein 5 antibody
    • Heat Shock 70kDa Protein 5 antibody
    • Heat shock protein family A (Hsp70) member 5 antibody
    • HEL S 89n antibody
    • Hsce70 antibody
    • HSPA 5 antibody
    • HSPA5 antibody
    • Immunoglobulin Heavy Chain Binding Protein antibody
    • Immunoglobulin heavy chain-binding protein antibody
    • mBiP antibody
    • MIF2 antibody
    • Sez7 antibody
    see all

Images

  • All lanes : Anti-GRP78 BiP antibody (ab227865) at 1/10000 dilution

    Lane 1 : Non-transfected HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
    Lane 2 : GRP78 BiP shRNA transfected HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 72 kDa



    7.5% SDS PAGE gel.

  • 4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for GRP78 BiP (green) using ab227865 at 1/500 dilution in ICC/IF.

    Nuclear counter stain: Hoechst 33342 (blue).

  • All lanes : Anti-GRP78 BiP antibody (ab227865) at 1/10000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 72 kDa



    7.5% SDS PAGE gel.

  • All lanes : Anti-GRP78 BiP antibody (ab227865) at 1/10000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma) whole cell extract
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 72 kDa



    7.5% SDS PAGE gel.

  • All lanes : Anti-GRP78 BiP antibody (ab227865) at 1/10000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate
    Lane 2 : JC whole cell lysate
    Lane 3 : BCL-1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 72 kDa



    7.5% SDS PAGE gel.

  • Paraffin-embedded mouse brain tissue stained for GRP78 BiP using ab227865 at 1/250 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat brain tissue stained for GRP78 BiP using ab227865 at 1/250 dilution in immunohistochemical analysis.

References

ab227865 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227865.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up