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    grp78-bip-antibody-ab230508.pdf

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Anti-GRP78 BiP antibody (ab230508)

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Western blot - Anti-GRP78 BiP antibody (ab230508)
  • Immunocytochemistry/ Immunofluorescence - Anti-GRP78 BiP antibody (ab230508)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody (ab230508)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody (ab230508)

Key features and details

  • Rabbit polyclonal to GRP78 BiP
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant human GRP78 BiP protein (Active) (ab78432)

View more associated products

Overview

  • Product name

    Anti-GRP78 BiP antibody
    See all GRP78 BiP primary antibodies
  • Description

    Rabbit polyclonal to GRP78 BiP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Monkey
  • Immunogen

    Synthetic peptide within Human GRP78 BiP (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P11021

  • Positive control

    • IHC-P: Human kidney and lung tissues. ICC/IF: HeLa cells. WB: HepG2, HeLa, Raw264.7 and mouse brain whole cell lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 30% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • ER

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HepG2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
    • Mouse brain tissue lysate - total protein (ab4022)
    • RAW 264.7 whole cell lysate (ab7187)
  • Recombinant Protein

    • Recombinant human GRP78 BiP protein (Active) (ab78432)

Applications

Our Abpromise guarantee covers the use of ab230508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
ICC/IF 1/100 - 1/500.
WB 1/10 - 1/100. Predicted molecular weight: 72 kDa.

Target

  • Function

    Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
  • Involvement in disease

    Autoantigen in rheumatoid arthritis.
  • Sequence similarities

    Belongs to the heat shock protein 70 family.
  • Cellular localization

    Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P11021 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3309 Human
    • Entrez Gene: 14828 Mouse
    • Omim: 138120 Human
    • SwissProt: P11021 Human
    • SwissProt: P20029 Mouse
    • Unigene: 743241 Human
    • Unigene: 330160 Mouse
    • Unigene: 470180 Mouse
    • Unigene: 474909 Mouse
    see all
  • Alternative names

    • 78 kDa glucose regulated protein antibody
    • 78 kDa glucose-regulated protein antibody
    • AL022860 antibody
    • AU019543 antibody
    • BIP antibody
    • D2Wsu141e antibody
    • D2Wsu17e antibody
    • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody
    • Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody
    • Epididymis secretory sperm binding protein Li 89n antibody
    • FLJ26106 antibody
    • Glucose Regulated Protein 78 antibody
    • Glucose Regulated Protein 78kDa antibody
    • GRP 78 antibody
    • GRP-78 antibody
    • GRP78 antibody
    • GRP78_HUMAN antibody
    • Heat shock 70 kDa protein 5 antibody
    • Heat Shock 70kDa Protein 5 antibody
    • Heat shock protein family A (Hsp70) member 5 antibody
    • HEL S 89n antibody
    • Hsce70 antibody
    • HSPA 5 antibody
    • HSPA5 antibody
    • Immunoglobulin Heavy Chain Binding Protein antibody
    • Immunoglobulin heavy chain-binding protein antibody
    • mBiP antibody
    • MIF2 antibody
    • Sez7 antibody
    see all

Images

  • Western blot - Anti-GRP78 BiP antibody (ab230508)
    Western blot - Anti-GRP78 BiP antibody (ab230508)
    All lanes : Anti-GRP78 BiP antibody (ab230508) at 1/500 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : Raw264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 4 : Mouse brain whole cell lysate

    Predicted band size: 72 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-GRP78 BiP antibody (ab230508)
    Immunocytochemistry/ Immunofluorescence - Anti-GRP78 BiP antibody (ab230508)

    Formalin-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GRP78 BiP (red) using ab230508 at 1/100 dilution and icubated with DyLight 594-conjugated secondary antibody PBS at room temperature in the dark.

    Nucelar counterstain: DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody (ab230508)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody (ab230508)

    Formalin-fixed, paraffin-embedded human lung tissue stained for GRP78 BiP using ab230508 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody (ab230508)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody (ab230508)

    Formalin-fixed, paraffin-embedded human kidney tissue stained for GRP78 BiP using ab230508 at 1/100 dilution in immunohistochemical analysis.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab230508? Please let us know so that we can cite the reference in this datasheet.

    ab230508 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab230508.
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