• Product name
  • Description
    Rabbit polyclonal to GRP78 BiP
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Chicken, Human
    Predicted to work with: Rat, Hamster, Xenopus laevis
  • Immunogen

    Synthetic peptide within Human GRP78 BiP aa 1-100. The exact sequence is proprietary.
    Database link: P11021

  • Positive control
    • HeLa cells, breast carcinoma.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab32618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 78 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100.


  • Function
    Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
  • Involvement in disease
    Autoantigen in rheumatoid arthritis.
  • Sequence similarities
    Belongs to the heat shock protein 70 family.
  • Cellular localization
    Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 78 kDa glucose regulated protein antibody
    • 78 kDa glucose-regulated protein antibody
    • AL022860 antibody
    • AU019543 antibody
    • BIP antibody
    • D2Wsu141e antibody
    • D2Wsu17e antibody
    • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody
    • Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody
    • Epididymis secretory sperm binding protein Li 89n antibody
    • FLJ26106 antibody
    • Glucose Regulated Protein 78kDa antibody
    • GRP 78 antibody
    • GRP-78 antibody
    • GRP78 antibody
    • GRP78_HUMAN antibody
    • Heat shock 70 kDa protein 5 antibody
    • Heat Shock 70kDa Protein 5 antibody
    • Heat shock protein family A (Hsp70) member 5 antibody
    • HEL S 89n antibody
    • Hsce70 antibody
    • HSPA 5 antibody
    • HSPA5 antibody
    • Immunoglobulin Heavy Chain Binding Protein antibody
    • Immunoglobulin heavy chain-binding protein antibody
    • mBiP antibody
    • MIF2 antibody
    • Sez7 antibody
    see all


  • All lanes : Anti-GRP78 BiP antibody (ab32618) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : CHO-K1 cell lysate Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 78 kDa
    Observed band size: 75 kDa (why is the actual band size different from the predicted?)

    Exposure time: 30 seconds

    The band observed at 75 kDa could potentially be a cleaved form of GRP78 BiP due to the presence of a 18 amino acid signal peptide.
  • ICC/IF image of ab32618 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32618, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • This image shows human breast carcinoma stained with ab32618 diluted 1/100.
  • Ab32618 staining Human normal liver parenchyma. Staining is localised to endoplasmic reticulum compartment.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be requi


This product has been referenced in:
  • Li X  et al. Cisplatin Enhances Hepatitis B Virus Replication and PGC-1a Expression through Endoplasmic Reticulum Stress. Sci Rep 8:3496 (2018). Read more (PubMed: 29472690) »
  • Zhao D  et al. Identification of Glucose-Regulated Protein 78 (GRP78) as a Receptor in BHK-21 Cells for Duck Tembusu Virus Infection. Front Microbiol 9:694 (2018). Read more (PubMed: 29692766) »

See all 29 Publications for this product

Customer reviews and Q&As

Vielen Dank fuer Ihre email.
Es tut mir leid zu hoeren, dass Sie Problem mit diesem Antikoerper haben.

Die Information bezueglich des defekten Lots GR67285 bezieht sich tatsaechlich nur auf dieses Lot.

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I am happy to hear that the new batch provides again the expected results. Also, I am very sorry for all the troubles encountered. We will replace all the vials of the old batch for you with a vial of the new batch. ...

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I have issued a free of charge replacement of a vial of the new batch of ab32618 with the order number x. To check the status of the order please contact our Customer Service team and reference this number. Read More

Thank you for your email. I am very sorry to hear that the replacement vial has not provided the expected results. We do not know the reason of this problem yet, but we will investigate this. Please do also keep us updated if you have new information i...

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I am very sorry that I missed the line about the protease inhibitors. I have myself never worked with stomach and I do not know how the lysate is susceptible to degradation. We will send you replacement vials, howev...

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Having reviewed this case, I would also appreciate if you can confirm some further details:

1.) I...

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I am sorry to hear you are experiencing difficulties with the new vials ofthe ab32618 (I guess it is ab32618 you ordered, as ab63618 does not correspond to the description). We have received no other negative fee...

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