Overview

  • Product name

    Anti-GRP78 BiP antibody - C-terminal
    See all GRP78 BiP primary antibodies
  • Description

    Rabbit polyclonal to GRP78 BiP - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, ICC, WB, IHC-Fr, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken, Cow, Pig, Xenopus laevis, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human GRP78 BiP aa 603-617 (C terminal).
    Sequence:

    EWLESHQDADIEDFK


    Database link: P11021

  • Positive control

    • Rat testis tissue lysate, A549, MCF7 and HeLa whole cell lysate (ab150035). Human lung cancer tissue, rat intestine and cerebellum tissue lysate. HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
ICC Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 72 kDa.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
  • Involvement in disease

    Autoantigen in rheumatoid arthritis.
  • Sequence similarities

    Belongs to the heat shock protein 70 family.
  • Cellular localization

    Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • 78 kDa glucose regulated protein antibody
    • 78 kDa glucose-regulated protein antibody
    • AL022860 antibody
    • AU019543 antibody
    • BIP antibody
    • D2Wsu141e antibody
    • D2Wsu17e antibody
    • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody
    • Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody
    • Epididymis secretory sperm binding protein Li 89n antibody
    • FLJ26106 antibody
    • Glucose Regulated Protein 78 antibody
    • Glucose Regulated Protein 78kDa antibody
    • GRP 78 antibody
    • GRP-78 antibody
    • GRP78 antibody
    • GRP78_HUMAN antibody
    • Heat shock 70 kDa protein 5 antibody
    • Heat Shock 70kDa Protein 5 antibody
    • Heat shock protein family A (Hsp70) member 5 antibody
    • HEL S 89n antibody
    • Hsce70 antibody
    • HSPA 5 antibody
    • HSPA5 antibody
    • Immunoglobulin Heavy Chain Binding Protein antibody
    • Immunoglobulin heavy chain-binding protein antibody
    • mBiP antibody
    • MIF2 antibody
    • Sez7 antibody
    see all

Images

  • All lanes : Anti-GRP78 BiP antibody - C-terminal (ab191023) at 0.5 µg/ml

    Lane 1 : Rat testis lysate
    Lane 2 : A549 lysate
    Lane 3 : MCF7 lysate
    Lane 4 : HeLa lysate

    Predicted band size: 72 kDa

  • Immunocytochemical analysis of HeLa cells, staining GRP78 BiP with ab191023 at 1 µg/ml.

  • Immunohistochemistry of paraffin-embedded Human lung cancer tissue labelling GRP78 BiP with ab191023 at 1 µg/ml.

  • Immunohistochemistry of paraffin-embedded rat cerebellum tissue labelling GRP78 BiP with ab191023 at 1 µg/ml.

  • Immunohistochemistry of frozen rat intestine tissue labelling GRP78 BiP with ab191023 at 1 µg/ml.

  • Immunohistochemistry of paraffin-embedded rat intestine tissue labelling GRP78 BiP with ab191023 at 1 µg/ml.

References

This product has been referenced in:

  • Sahebekhtiari N  et al. Deficiency of the mitochondrial sulfide regulator ETHE1 disturbs cell growth, glutathione level and causes proteome alterations outside mitochondria. Biochim Biophys Acta Mol Basis Dis 1865:126-135 (2019). Read more (PubMed: 30391543) »
  • Booth L  et al. PDE5 inhibitors enhance the lethality of pemetrexed through inhibition of multiple chaperone proteins and via the actions of cyclic GMP and nitric oxide. Oncotarget 8:1449-1468 (2017). ICC/IF . Read more (PubMed: 27903966) »
See all 11 Publications for this product

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