Recombinant Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4040(2)] to GRP78 BiP
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-GRP78 BiP antibody [EPR4040(2)]
See all GRP78 BiP primary antibodies -
Description
Rabbit monoclonal [EPR4040(2)] to GRP78 BiP -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for recommended treatment conditions and positive controls.
-
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HeLa, LnCaP, ECV-304, C6, NIH/3T3, PC-12, C6 (Rat glial tumor glial cell), RAW264.7, MCF7, 293T, U-87 MG, THP-1, MDA-MB-231 and HepG2 cell lysates; Human liver lysates; Mouse heart, lung, testis and liver lysates; Rat liver lysates. IHC-P: Human brain, ovarian cancer and testis tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4040(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 78 kDa (predicted molecular weight: 72 kDa).
|
|
IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. (Heat to 98oC, allow to cool for 10-20 minutes) The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
Notes |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 78 kDa (predicted molecular weight: 72 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. (Heat to 98oC, allow to cool for 10-20 minutes) The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
Target
-
Function
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. -
Involvement in disease
Autoantigen in rheumatoid arthritis. -
Sequence similarities
Belongs to the heat shock protein 70 family. -
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
Database links
- Entrez Gene: 3309 Human
- Entrez Gene: 14828 Mouse
- Entrez Gene: 25617 Rat
- Omim: 138120 Human
- SwissProt: P11021 Human
- SwissProt: P20029 Mouse
- SwissProt: P06761 Rat
- Unigene: 743241 Human
see all -
Alternative names
- 78 kDa glucose regulated protein antibody
- 78 kDa glucose-regulated protein antibody
- AL022860 antibody
see all
Images
-
All lanes : Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/1000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 3 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
Lane 5 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
Lane 6 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 7 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 8 : Human uterus lysates
Lane 9 : Human liver lysates
Lane 10 : Mouse heart lysates
Lane 11 : Mouse lung lysates
Lane 12 : Mouse testis lysates
Lane 13 : Mouse liver lysates
Lane 14 : Rat liver lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 72 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
Exposure time: 37 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian cancer tissue sections labeling GRP78 BiP with Purified ab108613 at 1:500 dilution (1.17 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylinwas used as a counterstain.
-
All lanes : Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa treated with 2.5 µg/ml tunicamycin for 24h whole cell lysates
Lane 3 : HUVEC (Human umbilical vein endothelial cell) whole cell lysates
Lane 4 : HUVEC treated with 10 µg/ml tunicamycin for 48h whole cell lysates
Lane 5 : Raw 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 6 : Raw 264.7 treated with 5 µg/ml tunicamycin for 18h whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 72 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
Exposure time: 3 seconds -
Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 0.1 µg/ml (purified) + C6 (Rat glial tumor glial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 72 kDaBlocking and diluting buffer: 5% NFDM/TBST.
-
Immunohistochemical analysis of paraffin-embedded Human testis tissue using unpurified ab108613 at 1/250
-
All lanes : Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/5000 dilution (purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10ug/ml
tunicamycin for 12 hours whole cell lysates
Lane 3 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : MCF-7 (Human breast adenocarcinoma epithelial cell) treated with 10ug/ml
tunicamycin for 48 hours whole cell lysates
Lane 5 : MEF (Mouse embryonic fibroblast) whole cell lysates
Lane 6 : MEF (Mouse embryonic fibroblast) treated with 10ug/ml tunicamycin for 48 hours whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 72 kDaBlocking and diluting buffer: 5% NFDM/TBST.
Tunicamycin induces the expression of GRP78 BiP reported by PMID 22859938Exposure time:
Lanes 1 and 2: 3 seconds
Lanes 3 and 4: 30 seconds
Lanes 5 and 6: 5 seconds -
Immunohistochemical analysis of paraffin-embedded Human brain tissue using unpurified ab108613 at 1/250
-
All lanes : Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/1000 dilution (Unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : LnCaP cell lysate
Lane 3 : ECV-304 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 72 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted? -
All lanes : Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/1000 dilution (Unpurified)
Lane 1 : C6 cell lysate
Lane 2 : NIH/3T3 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : RAW264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 72 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (48)
ab108613 has been referenced in 48 publications.
- Lai IC et al. Selenium Yeast and Fish Oil Combination Diminishes Cancer Stem Cell Traits and Reverses Cisplatin Resistance in A549 Sphere Cells. Nutrients 14:N/A (2022). PubMed: 35956408
- Otero A et al. Prion-Associated Neurodegeneration Causes Both Endoplasmic Reticulum Stress and Proteasome Impairment in a Murine Model of Spontaneous Disease. Int J Mol Sci 22:N/A (2021). PubMed: 33466523
- Echavarría-Consuegra L et al. Manipulation of the unfolded protein response: A pharmacological strategy against coronavirus infection. PLoS Pathog 17:e1009644 (2021). PubMed: 34138976
- Zhu Y et al. Paeoniflorin Effect of Schwann Cell-Derived Exosomes Ameliorates Dorsal Root Ganglion Neurons Apoptosis through IRE1α Pathway. Evid Based Complement Alternat Med 2021:6079305 (2021). PubMed: 34616478
- Fu T et al. The factors involved in the induction of neutrophil gelatinase-associated lipocalin overexpression in renal tubular epithelial cells under endoplasmic reticulum stress. J Physiol Pharmacol 72:N/A (2021). PubMed: 34642260