Recombinant Anti-GRP78 BiP antibody [EPR4040(2)] - BSA and Azide free (ab226042)
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- References
- Protocols
Overview
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Product name
Anti-GRP78 BiP antibody [EPR4040(2)] - BSA and Azide free
See all GRP78 BiP primary antibodies -
Description
Rabbit monoclonal [EPR4040(2)] to GRP78 BiP - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human GRP78 BiP aa 1-100. The exact sequence is proprietary.
(Peptide available asab176143) -
General notes
Ab226042 is the carrier-free version of ab108613. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab226042 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4040(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Immunohistochemistry kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab226042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. (Heat to 98°C, allow to cool for 10-20 minutes). The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
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| WB | Use at an assay dependent concentration. Detects a band of approximately 78 kDa (predicted molecular weight: 72 kDa).Can be blocked with GRP78 BiP peptide (ab176143). |
Target
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Function
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. -
Involvement in disease
Autoantigen in rheumatoid arthritis. -
Sequence similarities
Belongs to the heat shock protein 70 family. -
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 3309 Human
- Entrez Gene: 14828 Mouse
- Entrez Gene: 25617 Rat
- Omim: 138120 Human
- SwissProt: P11021 Human
- SwissProt: P20029 Mouse
- SwissProt: P06761 Rat
- Unigene: 743241 Human
see all -
Alternative names
- 78 kDa glucose regulated protein antibody
- 78 kDa glucose-regulated protein antibody
- AL022860 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4040(2)] - BSA and Azide free (ab226042)](https://www.abcam.com/ps/products/226/ab226042/Images/ab226042-3-ab1086137ab108613IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4040(2)] - BSA and Azide free (ab226042)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian cancer tissue sections labeling GRP78 BiP with Purified ab108613 at 1:500 dilution (1.17 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylinwas used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108613).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4040(2)] - BSA and Azide free (ab226042)](https://www.abcam.com/ps/products/226/ab226042/Images/ab226042-2-GRP78BiPPrimaryantibodiesab1086133.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4040(2)] - BSA and Azide free (ab226042)Immunohistochemical analysis of paraffin-embedded Human brain tissue using unpurified ab108613 at 1/250
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108613).
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4040(2)] - BSA and Azide free (ab226042)](https://www.abcam.com/ps/products/226/ab226042/Images/ab226042-1-GRP78BiPPrimaryantibodiesab1086134.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4040(2)] - BSA and Azide free (ab226042)Immunohistochemical analysis of paraffin-embedded Human testis tissue using unpurified ab108613 at 1/250
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108613).
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Datasheets and documents
References
ab226042 has not yet been referenced specifically in any publications.
