Product nameAnti-GRP94 antibody
See all GRP94 primary antibodies
DescriptionRabbit polyclonal to GRP94
Tested applicationsSuitable for: WB, IP, IHC-P, IHC-Fr, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Cow, Dog, Human
- Rat Brain Tissue Extract.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab13509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 94 kDa. 2µg/mL of ab13509 detects of Grp94 in 20µg of Rat Brain Tissue Extract using colorimetric analysis. 1/2000 for ECL detection.|
|IP||Use a concentration of 5 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionMolecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
Sequence similaritiesBelongs to the heat shock protein 90 family.
Cellular localizationEndoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 94 kDa glucose regulated protein antibody
- 94 kDa glucose-regulated protein antibody
- ECGP antibody
All lanes : Anti-GRP94 antibody (ab13509) at 1/1000 dilution
Lane 1 : Molecular weight ladder
Lane 2 : Lysates prepared from rat brain
Lane 3 : Lysates prepared from mouse brain
Lane 4 : Lysates prepared from rat liver microsomes
Lane 5 : Lysates prepared from mouse liver microsomes
Lane 6 : Lysates prepared from human liver microsomes
Predicted band size: 94 kDa
ICC/IF image of ab13509 stained rat PC12 cells. The cells were 4% PFA and 100% methanol fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13509, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
GRP94 was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Rabbit polyclonal to GRP94 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab13509.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 100kDa: GRP94
This product has been referenced in:
- Andreani V et al. Cochaperone Mzb1 is a key effector of Blimp1 in plasma cell differentiation and ß1-integrin function. Proc Natl Acad Sci U S A 115:E9630-E9639 (2018). Read more (PubMed: 30257949) »
- Rosenbaum M et al. MZB1 is a GRP94 cochaperone that enables proper immunoglobulin heavy chain biosynthesis upon ER stress. Genes Dev 28:1165-78 (2014). IP ; Mouse . Read more (PubMed: 24888588) »