Overview

  • Product name
  • Description
    Rabbit polyclonal to GRP94
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Hamster, Human, Monkey
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (Human) (Internal sequence).

Properties

Applications

Our Abpromise guarantee covers the use of ab18055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
ICC/IF
WB
  • Application notes

    ICC: 1/100 - 1/400.
    IF: 1/100 - 1/400.
    WB: 1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    Works in ICC/IF with methanol-fixed and 4% paraformaldehyde fixed cells.
  • Target

    • Function
      Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
    • Sequence similarities
      Belongs to the heat shock protein 90 family.
    • Cellular localization
      Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • 94 kDa glucose regulated protein antibody
      • 94 kDa glucose-regulated protein antibody
      • ECGP antibody
      • Endoplasmin antibody
      • Endothelial cell (HBMEC) glycoprotein antibody
      • ENPL_HUMAN antibody
      • Glucose regulated protein 94kDa antibody
      • gp96 antibody
      • gp96 homolog antibody
      • GRP 94 antibody
      • GRP-94 antibody
      • Heat shock protein 90 kDa beta member 1 antibody
      • heat shock protein 90kDa beta (Grp94), member 1 antibody
      • Heat shock protein, 90 kDa, beta, 1 antibody
      • HSP90B1 antibody
      • Stress inducible tumor rejection antigen GP96 antibody
      • TRA1 antibody
      • tumor rejection antigen (gp96) 1 antibody
      • Tumor rejection antigen 1 antibody
      • Tumor rejection antigen gp96 antibody
      • Tumor rejection antigen-1 (gp96) antibody
      see all

    Images

    • Methanol-fixed HEK293 cells stained at 1/200 with ab18055 followed by FITC conjugated secondary.

    • Western blot of soluble fraction of monkey brain stained at 1/1000 with ab18055, visualised with HRP-conjugated secondary and ECL. Western blot of soluble fraction of monkey brain stained at 1/1000 with ab18055, visualised with HRP-conjugated secondary and ECL.

    References

    This product has been referenced in:
    • Whitmore LC  et al. A Common Genetic Variant in TLR1 Enhances Human Neutrophil Priming and Impacts Length of Intensive Care Stay in Pediatric Sepsis. J Immunol 196:1376-86 (2016). WB, IP ; Human . Read more (PubMed: 26729809) »
    • Kim N  et al. Interferon-inducible protein SCOTIN interferes with HCV replication through the autolysosomal degradation of NS5A. Nat Commun 7:10631 (2016). Read more (PubMed: 26868272) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    Wir haben leider aktuell keinen validierten anti-Housekeeping Protein Antikörper der nur humane Proteine erkennt. Wir wissen, dass der ab3369 Click here (or use the following: https://www.abcam.com/index.html?datasheet=3369) gegen MRP1 nicht mit der Maus reagiert aber mit dem humanen Protein. Ich weiss jedoch nicht ob MRP1 als housekeeping in generell oder in Ihrem Fall geeignet ist. Auch der anti Cyt C und anti Golgi Antikörper ab76107 und ab58826 haben die Information, nicht mit der Maus zu reagieren. Bei diesen zwei Antikörpern würde ich jedoch gerne nochmals mit dem Labor abklären, ob wir die Nicht-Reaktion mit der Maus garantieren können oder ob sie eher indikativ ist. Bitte lassen Sie mich wissen, ob einer dieser Antikörper Sie interessiert, und ich werde die Information absichern. Click here (or use the following: https://www.abcam.com/index.html?datasheet=76107). Click here (or use the following: https://www.abcam.com/index.html?datasheet=58826). Ich habe Ihnen auch die Excel Liste mit den Antikörpern welche mit dem humanen Protein jedoch nicht mit Maus reagieren in den Anhang getan. Falls eines dieser Zielprotein Sie interessiert, werde ich gerne die Nicht-Reaktion absichern für Sie.  

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (huh-7)
    Loading amount
    15000 cells
    Specification
    huh-7
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Nov 13 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Hamster Cell lysate - whole cell (Chinese Hamster Ovary cell line)
    Loading amount
    50 µg
    Specification
    Chinese Hamster Ovary cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%

    Abcam user community

    Verified customer

    Submitted Aug 11 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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