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    grp94-antibody-ab18055.pdf

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Tags & Cell Markers Subcellular Markers Organelles ER
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Anti-GRP94 antibody (ab18055)

  • Datasheet
Reviews (2)Q&A (1)References (6)

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Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab18055)
  • Western blot - Anti-GRP94 antibody (ab18055)

Key features and details

  • Rabbit polyclonal to GRP94
  • Suitable for: ICC/IF, WB
  • Reacts with: Hamster, Human, Monkey
  • Isotype: IgG

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Overview

  • Product name

    Anti-GRP94 antibody
    See all GRP94 primary antibodies
  • Description

    Rabbit polyclonal to GRP94
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Hamster, Human, Monkey
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (Human) (Internal sequence).

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituent: Whole serum
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • ER
    • Signal Transduction
    • Protein Trafficking
    • ER Proteins
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human GRP94 protein (ab87144)

Applications

Our Abpromise guarantee covers the use of ab18055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notes

    ICC: 1/100 - 1/400.
    IF: 1/100 - 1/400.
    WB: 1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    Works in ICC/IF with methanol-fixed and 4% paraformaldehyde fixed cells.
  • Target

    • Function

      Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
    • Sequence similarities

      Belongs to the heat shock protein 90 family.
    • Cellular localization

      Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Target information above from: UniProt accession P14625 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7184 Human
      • Omim: 191175 Human
      • SwissProt: P14625 Human
      • Unigene: 192374 Human
      • Alternative names

        • 94 kDa glucose regulated protein antibody
        • 94 kDa glucose-regulated protein antibody
        • ECGP antibody
        • Endoplasmin antibody
        • Endothelial cell (HBMEC) glycoprotein antibody
        • ENPL_HUMAN antibody
        • Glucose regulated protein 94kDa antibody
        • gp96 antibody
        • gp96 homolog antibody
        • GRP 94 antibody
        • GRP-94 antibody
        • Heat shock protein 90 kDa beta member 1 antibody
        • heat shock protein 90kDa beta (Grp94), member 1 antibody
        • Heat shock protein, 90 kDa, beta, 1 antibody
        • HSP90B1 antibody
        • Stress inducible tumor rejection antigen GP96 antibody
        • TRA1 antibody
        • tumor rejection antigen (gp96) 1 antibody
        • Tumor rejection antigen 1 antibody
        • Tumor rejection antigen gp96 antibody
        • Tumor rejection antigen-1 (gp96) antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab18055)
        Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab18055)

        Methanol-fixed HEK293 cells stained at 1/200 with ab18055 followed by FITC conjugated secondary.

      • Western blot - Anti-GRP94 antibody (ab18055)
        Western blot - Anti-GRP94 antibody (ab18055)
        Western blot of soluble fraction of monkey brain stained at 1/1000 with ab18055, visualised with HRP-conjugated secondary and ECL. Western blot of soluble fraction of monkey brain stained at 1/1000 with ab18055, visualised with HRP-conjugated secondary and ECL.

      Protocols

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (6)

      Publishing research using ab18055? Please let us know so that we can cite the reference in this datasheet.

      ab18055 has been referenced in 6 publications.

      • Marrugal Á  et al. Impact of Heat Shock Protein 90 Inhibition on the Proteomic Profile of Lung Adenocarcinoma as Measured by Two-Dimensional Electrophoresis Coupled with Mass Spectrometry. Cells 8:N/A (2019). PubMed: 31370342
      • Whitmore LC  et al. A Common Genetic Variant in TLR1 Enhances Human Neutrophil Priming and Impacts Length of Intensive Care Stay in Pediatric Sepsis. J Immunol 196:1376-86 (2016). WB, IP ; Human . PubMed: 26729809
      • Kim N  et al. Interferon-inducible protein SCOTIN interferes with HCV replication through the autolysosomal degradation of NS5A. Nat Commun 7:10631 (2016). PubMed: 26868272
      • Weston CJ  et al. Cellular localization and trafficking of vascular adhesion protein-1 as revealed by an N-terminal GFP fusion protein. J Neural Transm 120:951-61 (2013). ICC/IF ; Human . PubMed: 23474851
      • Mulya A  et al. Apolipoprotein M expression increases the size of nascent pre beta HDL formed by ATP binding cassette transporter A1. J Lipid Res 51:514-24 (2010). ICC/IF ; Human . PubMed: 19767535
      • Kang BY  et al. Differential gene expression profiling in HELLP syndrome placentas. Reprod Sci 15:285-94 (2008). PubMed: 18421023

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-3 of 3 Abreviews or Q&A

      Question

      Wir sind auf der Suche nach einem Housekeeping Protein, das wir in Western Blot Analysen detektieren wollen, dass aber außschliesslich das humane Protein detektiert und nicht an Mausprotein bindet, das ja auch in der Proteinisolation enthalten ist. Vielelicht haben Sie ja eine Idee, welches Protein dafür in Frage kommt. Vielen Dank und mit freundlichen Grüßen

      Read More

      Abcam community

      Verified customer

      Asked on Oct 24 2011

      Answer

      Wir haben leider aktuell keinen validierten anti-Housekeeping Protein Antikörper der nur humane Proteine erkennt. Wir wissen, dass der ab3369 Click here (or use the following: https://www.abcam.com/MRP1-antibody-QCRL1-ab3369.html) gegen MRP1 nicht mit der Maus reagiert aber mit dem humanen Protein. Ich weiss jedoch nicht ob MRP1 als housekeeping in generell oder in Ihrem Fall geeignet ist. Auch der anti Cyt C und anti Golgi Antikörper ab76107 und ab58826 haben die Information, nicht mit der Maus zu reagieren. Bei diesen zwei Antikörpern würde ich jedoch gerne nochmals mit dem Labor abklären, ob wir die Nicht-Reaktion mit der Maus garantieren können oder ob sie eher indikativ ist. Bitte lassen Sie mich wissen, ob einer dieser Antikörper Sie interessiert, und ich werde die Information absichern. Click here (or use the following: https://www.abcam.com/Cytochrome-C-antibody-EP1326-80-5-ab76107.html). Click here (or use the following: https://www.abcam.com/Golgi-Complex-antibody-AE-6-ab58826.html). Ich habe Ihnen auch die Excel Liste mit den Antikörpern welche mit dem humanen Protein jedoch nicht mit Maus reagieren in den Anhang getan. Falls eines dieser Zielprotein Sie interessiert, werde ich gerne die Nicht-Reaktion absichern für Sie.  

      Read More

      Abcam Scientific Support

      Answered on Oct 24 2011

      Western blot abreview for Anti-GRP94 antibody - ER Marker

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (huh-7)
      Loading amount
      15000 cells
      Specification
      huh-7
      Gel Running Conditions
      Reduced Denaturing (12%)
      Blocking step
      Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 13 2008

      Western blot abreview for Anti-GRP94 antibody - ER Marker

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Hamster Cell lysate - whole cell (Chinese Hamster Ovary cell line)
      Loading amount
      50 µg
      Specification
      Chinese Hamster Ovary cell line
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 11 2006

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