Overview

  • Product name
    Anti-GRP94 antibody
    See all GRP94 primary antibodies
  • Description
    Rabbit polyclonal to GRP94
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Hamster, Human, Monkey
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (Human) (Internal sequence).

Properties

Applications

Our Abpromise guarantee covers the use of ab18055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
ICC/IF
WB
  • Application notes

    ICC: 1/100 - 1/400.
    IF: 1/100 - 1/400.
    WB: 1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    Works in ICC/IF with methanol-fixed and 4% paraformaldehyde fixed cells.

    • Target

    • Function
      Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
    • Sequence similarities
      Belongs to the heat shock protein 90 family.
    • Cellular localization
      Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
      • Alternative names
        • 94 kDa glucose regulated protein antibody
        • 94 kDa glucose-regulated protein antibody
        • ECGP antibody
        • Endoplasmin antibody
        • Endothelial cell (HBMEC) glycoprotein antibody
        • ENPL_HUMAN antibody
        • Glucose regulated protein 94kDa antibody
        • gp96 antibody
        • gp96 homolog antibody
        • GRP 94 antibody
        • GRP-94 antibody
        • Heat shock protein 90 kDa beta member 1 antibody
        • heat shock protein 90kDa beta (Grp94), member 1 antibody
        • Heat shock protein, 90 kDa, beta, 1 antibody
        • HSP90B1 antibody
        • Stress inducible tumor rejection antigen GP96 antibody
        • TRA1 antibody
        • tumor rejection antigen (gp96) 1 antibody
        • Tumor rejection antigen 1 antibody
        • Tumor rejection antigen gp96 antibody
        • Tumor rejection antigen-1 (gp96) antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab18055)
        Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab18055)

        Methanol-fixed HEK293 cells stained at 1/200 with ab18055 followed by FITC conjugated secondary.

      • Western blot - Anti-GRP94 antibody (ab18055)
        Western blot - Anti-GRP94 antibody (ab18055)
        Western blot of soluble fraction of monkey brain stained at 1/1000 with ab18055, visualised with HRP-conjugated secondary and ECL. Western blot of soluble fraction of monkey brain stained at 1/1000 with ab18055, visualised with HRP-conjugated secondary and ECL.

      References

      This product has been referenced in:
      • Whitmore LC  et al. A Common Genetic Variant in TLR1 Enhances Human Neutrophil Priming and Impacts Length of Intensive Care Stay in Pediatric Sepsis. J Immunol 196:1376-86 (2016). WB, IP ; Human . Read more (PubMed: 26729809) »
      • Kim N  et al. Interferon-inducible protein SCOTIN interferes with HCV replication through the autolysosomal degradation of NS5A. Nat Commun 7:10631 (2016). Read more (PubMed: 26868272) »

      See all 5 Publications for this product

      Publishing research using ab18055? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Submit a review Submit a question

      3 reviews or Q&A

      10
      Votes: Highest First

      Wir sind auf der Suche nach einem Housekeeping Protein, das wir in Western Blot Analysen detektieren wollen, dass aber außschliesslich das humane Protein detektiert und nicht an Mausprotein bindet, das ja auch in der Proteinisolation enthalten ist. Vie...

      Read More

      Wir haben leider aktuell keinen validierten anti-Housekeeping Protein Antikörper der nur humane Proteine erkennt. Wir wissen, dass der ab3369 Click here (or use the following: https://www.abcam.com/index.html?datasheet=3369) gegen MRP1 nicht mit der Ma...

      Read More

      Western blot abreview for Anti-GRP94 antibody - ER Marker

       Good
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (huh-7)
      Loading amount
      15000 cells
      Specification
      huh-7
      Gel Running Conditions
      Reduced Denaturing (12%)
      Blocking step
      Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More
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      Username

      Abcam user community

      Verified customer

      Submitted Nov 13 2008

      Western blot abreview for Anti-GRP94 antibody - ER Marker

       Average
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Western blot
      Sample
      Hamster Cell lysate - whole cell (Chinese Hamster Ovary cell line)
      Loading amount
      50 µg
      Specification
      Chinese Hamster Ovary cell line
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
      Read More
      abreview image
      Username

      Abcam user community

      Verified customer

      Submitted Aug 11 2006

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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