Anti-GRP94 antibody (ab18055)
Key features and details
- Rabbit polyclonal to GRP94
- Suitable for: ICC/IF, WB
- Reacts with: Human, Monkey
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GRP94 antibody
See all GRP94 primary antibodies -
Description
Rabbit polyclonal to GRP94 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human, Monkey
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide (Human) (Internal sequence).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| WB | (2) |
| Notes |
|---|
Application notes
ICC: 1/100 - 1/400.
IF: 1/100 - 1/400.
WB: 1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Works in ICC/IF with methanol-fixed and 4% paraformaldehyde fixed cells.
Target
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Function
Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. -
Sequence similarities
Belongs to the heat shock protein 90 family. -
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 7184 Human
- Omim: 191175 Human
- SwissProt: P14625 Human
- Unigene: 192374 Human
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Alternative names
- 94 kDa glucose regulated protein antibody
- 94 kDa glucose-regulated protein antibody
- ECGP antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab18055)
Methanol-fixed HEK293 cells stained at 1/200 with ab18055 followed by FITC conjugated secondary.
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Western blot - Anti-GRP94 antibody (ab18055)Western blot of soluble fraction of monkey brain stained at 1/1000 with ab18055, visualised with HRP-conjugated secondary and ECL. Western blot of soluble fraction of monkey brain stained at 1/1000 with ab18055, visualised with HRP-conjugated secondary and ECL.
Protocols
Datasheets and documents
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Datasheet download
References (6)
ab18055 has been referenced in 6 publications.
- Marrugal Á et al. Impact of Heat Shock Protein 90 Inhibition on the Proteomic Profile of Lung Adenocarcinoma as Measured by Two-Dimensional Electrophoresis Coupled with Mass Spectrometry. Cells 8:N/A (2019). PubMed: 31370342
- Whitmore LC et al. A Common Genetic Variant in TLR1 Enhances Human Neutrophil Priming and Impacts Length of Intensive Care Stay in Pediatric Sepsis. J Immunol 196:1376-86 (2016). WB, IP ; Human . PubMed: 26729809
- Kim N et al. Interferon-inducible protein SCOTIN interferes with HCV replication through the autolysosomal degradation of NS5A. Nat Commun 7:10631 (2016). PubMed: 26868272
- Weston CJ et al. Cellular localization and trafficking of vascular adhesion protein-1 as revealed by an N-terminal GFP fusion protein. J Neural Transm 120:951-61 (2013). ICC/IF ; Human . PubMed: 23474851
- Mulya A et al. Apolipoprotein M expression increases the size of nascent pre beta HDL formed by ATP binding cassette transporter A1. J Lipid Res 51:514-24 (2010). ICC/IF ; Human . PubMed: 19767535
- Kang BY et al. Differential gene expression profiling in HELLP syndrome placentas. Reprod Sci 15:285-94 (2008). PubMed: 18421023
