Overview

  • Product name

  • Description

    Rabbit polyclonal to GRP94
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse GRP94 (C terminal). Corresponding to the sequence near the C-terminus.
    Database link: P08113

  • Positive control

    • WB: Rat brain cell lysate. ICC/IF: Heat shocked HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab223371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/120.
WB 1/1000. Detects a band of approximately 94-100 kDa (predicted molecular weight: 93 kDa).

Target

  • Function

    Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
  • Sequence similarities

    Belongs to the heat shock protein 90 family.
  • Cellular localization

    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • 94 kDa glucose regulated protein antibody
    • 94 kDa glucose-regulated protein antibody
    • ECGP antibody
    • Endoplasmin antibody
    • Endothelial cell (HBMEC) glycoprotein antibody
    • ENPL_HUMAN antibody
    • Glucose regulated protein 94kDa antibody
    • gp96 antibody
    • gp96 homolog antibody
    • GRP 94 antibody
    • GRP-94 antibody
    • Heat shock protein 90 kDa beta member 1 antibody
    • heat shock protein 90kDa beta (Grp94), member 1 antibody
    • Heat shock protein, 90 kDa, beta, 1 antibody
    • HSP90B1 antibody
    • Stress inducible tumor rejection antigen GP96 antibody
    • TRA1 antibody
    • tumor rejection antigen (gp96) 1 antibody
    • Tumor rejection antigen 1 antibody
    • Tumor rejection antigen gp96 antibody
    • Tumor rejection antigen-1 (gp96) antibody
    see all

Images

  • 2% formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GRP94 (red) using ab223371 at 1/120 dilution in ICC/IF. Secondary Antibody: Goat Anti-Rabbit APC at 1/200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at room temperature.

    (A) DAPI nuclear stain (B) ab223371 (C) Merge.

  • Anti-GRP94 antibody (ab223371) at 1/250 dilution + Rat brain cell lysate at 20 µg

    Predicted band size: 93 kDa
    Observed band size: 50,75,94-100 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes


    Blocking buffer: 5% milk in TBST.

    Color Development: TMB solution.

    Primary antibody was incubated for 1 hour at room temperature.

  • 2% formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GRP94 (green) using ab223371 at 1/120 dilution in ICC/IF. Secondary Antibody: Goat Anti-Rabbit FITC at 1/200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at room temperature.

    (A) DAPI nuclear stain (B) ab223371 (C) Merge.

References

ab223371 has not yet been referenced specifically in any publications.

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