Overview

  • Product name

  • Description

    Rabbit polyclonal to GRP94
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Dog, Pig, Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human GRP94 (internal sequence). The exact sequence is proprietary.
    Database link: P14625

  • Positive control

    • WB: NIH/3T3, NCI-H1299 and HeLa S3 whole cell lysates. IP: HeLa whole cell extract. IHC-P: SCM-1 xenograft tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.
WB 1/1000 - 1/10000. Predicted molecular weight: 92 kDa.

Target

  • Function

    Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
  • Sequence similarities

    Belongs to the heat shock protein 90 family.
  • Cellular localization

    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • 94 kDa glucose regulated protein antibody
    • 94 kDa glucose-regulated protein antibody
    • ECGP antibody
    • Endoplasmin antibody
    • Endothelial cell (HBMEC) glycoprotein antibody
    • ENPL_HUMAN antibody
    • Glucose regulated protein 94kDa antibody
    • gp96 antibody
    • gp96 homolog antibody
    • GRP 94 antibody
    • GRP-94 antibody
    • Heat shock protein 90 kDa beta member 1 antibody
    • heat shock protein 90kDa beta (Grp94), member 1 antibody
    • Heat shock protein, 90 kDa, beta, 1 antibody
    • HSP90B1 antibody
    • Stress inducible tumor rejection antigen GP96 antibody
    • TRA1 antibody
    • tumor rejection antigen (gp96) 1 antibody
    • Tumor rejection antigen 1 antibody
    • Tumor rejection antigen gp96 antibody
    • Tumor rejection antigen-1 (gp96) antibody
    see all

Images

  • All lanes : Anti-GRP94 antibody (ab227293) at 1/2000 dilution

    Lane 1 : WT 293T cell extracts
    Lane 2 : GRP94 Knockout 293T cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 92 kDa

  • Anti-GRP94 antibody (ab227293) at 1/5000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

    Predicted band size: 92 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-GRP94 antibody (ab227293) at 1/1000 dilution

    Lane 1 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : HeLa S3 (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 92 kDa



    7.5% SDS-PAGE gel.

  • GRP94 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 5 μg ab227293. Western blot was performed from the immunoprecipitate using ab227293.

    Lane 1: HeLa whole cell extract (Input).

    Lane 2: Control IgG instead of ab227293 in HeLa whole cell extract.

    Lane 3: ab227293 IP in HeLa whole cell extract.

  • Paraffin-embedded SCM-1 xenograft tissue stained for GRP94 using ab227293 at 1/500 dilution in immunohistochemical analysis.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GRP94 (green) using ab227293 at 1/500 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342 (bue).

References

ab227293 has not yet been referenced specifically in any publications.

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