Overview

  • Product name

  • Description

    Rabbit polyclonal to GRP94
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Monkey
  • Immunogen

    Synthetic peptide corresponding to Human GRP94 aa 460-487.
    Sequence:

    IRKKLVRKTLDMIKKIADDKYNDTFWKE


    Database link: P14625

  • Positive control

    • WB: C2C12, MCF7 and HeLa whole cell lysate. IHC-P: Human small intestine tissue. FC: A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab230650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 92 kDa.
IHC-P 1/100.
Flow Cyt 1/10 - 1/50.

Target

  • Function

    Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
  • Sequence similarities

    Belongs to the heat shock protein 90 family.
  • Cellular localization

    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • 94 kDa glucose regulated protein antibody
    • 94 kDa glucose-regulated protein antibody
    • ECGP antibody
    • Endoplasmin antibody
    • Endothelial cell (HBMEC) glycoprotein antibody
    • ENPL_HUMAN antibody
    • Glucose regulated protein 94kDa antibody
    • gp96 antibody
    • gp96 homolog antibody
    • GRP 94 antibody
    • GRP-94 antibody
    • Heat shock protein 90 kDa beta member 1 antibody
    • heat shock protein 90kDa beta (Grp94), member 1 antibody
    • Heat shock protein, 90 kDa, beta, 1 antibody
    • HSP90B1 antibody
    • Stress inducible tumor rejection antigen GP96 antibody
    • TRA1 antibody
    • tumor rejection antigen (gp96) 1 antibody
    • Tumor rejection antigen 1 antibody
    • Tumor rejection antigen gp96 antibody
    • Tumor rejection antigen-1 (gp96) antibody
    see all

Images

  • All lanes : Anti-GRP94 antibody (ab230650) at 1/1000 dilution

    Lane 1 : C2C12 (mouse myoblast cell line) whole cell lysate
    Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Predicted band size: 92 kDa

  • Formalin-fixed, paraffin-embedded human small intestine tissue stained for GRP94 using ab230650 at 1/100 dilution in immunohistochemical analysis.

  • Flow cytometric analysis of A549 (human lung carcinoma cell line) cell line labeling GRP94 with ab230650 at 1/10 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab230650 has not yet been referenced specifically in any publications.

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