Key features and details
- Rabbit polyclonal to GRP94
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Product nameAnti-GRP94 antibody
See all GRP94 primary antibodies
DescriptionRabbit polyclonal to GRP94
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Monkey
- WB: C2C12, MCF7 and HeLa whole cell lysate. IHC-P: Human small intestine tissue. Flow Cyt: A549 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab230650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 92 kDa.|
|Flow Cyt||1/10 - 1/50.|
FunctionMolecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
Sequence similaritiesBelongs to the heat shock protein 90 family.
Cellular localizationEndoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 94 kDa glucose regulated protein antibody
- 94 kDa glucose-regulated protein antibody
- ECGP antibody
All lanes : Anti-GRP94 antibody (ab230650) at 1/1000 dilution
Lane 1 : C2C12 (mouse myoblast cell line) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Predicted band size: 92 kDa
Formalin-fixed, paraffin-embedded human small intestine tissue stained for GRP94 using ab230650 at 1/100 dilution in immunohistochemical analysis.
Flow cytometric analysis of A549 (human lung carcinoma cell line) cell line labeling GRP94 with ab230650 at 1/10 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230650 has not yet been referenced specifically in any publications.