• Product name

  • Description

    Rabbit polyclonal to GRP94
  • Host species

  • Tested applications

    Suitable for: IHC-P, WB, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human, Chinese hamster
    Predicted to work with: Rat, Chicken, Cow, Pig, Monkey
  • Immunogen

    Synthetic peptide within GRP94 aa 16-43 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P14625

  • Positive control

    • IHC-P: Human testis and liver tissues. ICC/IF: NCI-H460 cells. WB: HepG2, CHO-K1, K562 and HeLa whole cell lysate (ab150035); Mouse liver lysate. Flow Cyt: K562 cells.



Our Abpromise guarantee covers the use of ab230842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
WB 1/1000. Predicted molecular weight: 92 kDa.
Flow Cyt 1/10 - 1/50.
ICC/IF 1/10 - 1/50.


  • Function

    Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
  • Sequence similarities

    Belongs to the heat shock protein 90 family.
  • Cellular localization

    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • 94 kDa glucose regulated protein antibody
    • 94 kDa glucose-regulated protein antibody
    • ECGP antibody
    • Endoplasmin antibody
    • Endothelial cell (HBMEC) glycoprotein antibody
    • ENPL_HUMAN antibody
    • Glucose regulated protein 94kDa antibody
    • gp96 antibody
    • gp96 homolog antibody
    • GRP 94 antibody
    • GRP-94 antibody
    • Heat shock protein 90 kDa beta member 1 antibody
    • heat shock protein 90kDa beta (Grp94), member 1 antibody
    • Heat shock protein, 90 kDa, beta, 1 antibody
    • HSP90B1 antibody
    • Stress inducible tumor rejection antigen GP96 antibody
    • TRA1 antibody
    • tumor rejection antigen (gp96) 1 antibody
    • Tumor rejection antigen 1 antibody
    • Tumor rejection antigen gp96 antibody
    • Tumor rejection antigen-1 (gp96) antibody
    see all


  • All lanes : Anti-GRP94 antibody (ab230842) at 1/1000 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
    Lane 2 : Mouse liver lysate
    Lane 3 : CHO-K1 (chinese hamster ovary cell line) cell lysate
    Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
    Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 92 kDa

  • Formalin-fixed, paraffin-embedded human testis tissue stained for GRP94 using ab230842 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human liver tissue stained for GRP94 using ab230842 at 1/100 dilution in immunohistochemical analysis.

  • NCI-H460 (human lung cancer cell line) cells stained for GRP94 (green) using ab230842 at 1/10 dilution in confocal ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG secondary antibody. DAPI was used to stain the cell nuclear (blue).

  • Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cell line labeling GRP94 with ab230842 at 1/10 dilution (green) compared with a negative control cell (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


ab230842 has not yet been referenced specifically in any publications.

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