Key features and details
- Rabbit polyclonal to GRP94
- Suitable for: IHC-P, WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human, Chinese hamster
- Isotype: IgG
Product nameAnti-GRP94 antibody
See all GRP94 primary antibodies
DescriptionRabbit polyclonal to GRP94
Tested applicationsSuitable for: IHC-P, WB, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Mouse, Human, Chinese hamster
Predicted to work with: Rat, Chicken, Cow, Pig, Monkey
Synthetic peptide within GRP94 aa 16-43 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P14625
- IHC-P: Human testis and liver tissues. ICC/IF: NCI-H460 cells. WB: HepG2, CHO-K1, K562 and HeLa whole cell lysate (ab150035); Mouse liver lysate. Flow Cyt: K562 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab230842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 92 kDa.|
|Flow Cyt||1/10 - 1/50.|
|ICC/IF||1/10 - 1/50.|
FunctionMolecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
Sequence similaritiesBelongs to the heat shock protein 90 family.
Cellular localizationEndoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 94 kDa glucose regulated protein antibody
- 94 kDa glucose-regulated protein antibody
- ECGP antibody
All lanes : Anti-GRP94 antibody (ab230842) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : Mouse liver lysate
Lane 3 : CHO-K1 (chinese hamster ovary cell line) cell lysate
Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 92 kDa
Formalin-fixed, paraffin-embedded human testis tissue stained for GRP94 using ab230842 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human liver tissue stained for GRP94 using ab230842 at 1/100 dilution in immunohistochemical analysis.
NCI-H460 (human lung cancer cell line) cells stained for GRP94 (green) using ab230842 at 1/10 dilution in confocal ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG secondary antibody. DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cell line labeling GRP94 with ab230842 at 1/10 dilution (green) compared with a negative control cell (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230842 has not yet been referenced specifically in any publications.