Anti-GRP94 antibody (ab52031)
Key features and details
- Goat polyclonal to GRP94
- Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GRP94 antibody
See all GRP94 primary antibodies -
Description
Goat polyclonal to GRP94 -
Host species
Goat -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Dog, Cynomolgus monkey, Syrian hamster, Orangutan -
Immunogen
Synthetic peptide:
C-KEGVKFDESEKTKE
, corresponding to internal sequence amino acids 593-606 of Human GRP94 -
Positive control
- WB: NIH-3T3, HEK 293, A549, HeLa and U-2OS cell lysates; IHC: Human liver and uterus tissue. ICC/IF: A431 and HeLa cells. Flow Cyt (intra): A431 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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IHC-P |
Use a concentration of 3 - 5 µg/ml.
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB | (1) |
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
IHC-P
Use a concentration of 3 - 5 µg/ml. |
ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. -
Sequence similarities
Belongs to the heat shock protein 90 family. -
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 7184 Human
- Entrez Gene: 22027 Mouse
- Entrez Gene: 100009456 Rabbit
- Entrez Gene: 362862 Rat
- Omim: 191175 Human
- SwissProt: P14625 Human
- SwissProt: P08113 Mouse
- SwissProt: O18750 Rabbit
see all -
Alternative names
- 94 kDa glucose regulated protein antibody
- 94 kDa glucose-regulated protein antibody
- ECGP antibody
see all
Images
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Lanes 1-3 : Anti-GRP94 antibody (ab52031) at 0.1 µg/ml
Lane 4 : Anti-GRP94 antibody (ab52031) at 0.3 µg/ml
Lane 1 : U2OS cell lysate
Lane 2 : HEK293 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A594 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 92 kDa -
Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation with ab52031 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing Endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab52031 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Anti-GRP94 antibody (ab52031) at 0.1 µg/ml + U-2OS cell lysate
Predicted band size: 92 kDa -
Anti-GRP94 antibody (ab52031) at 0.1 µg/ml + NIH-3T3 cell lysate
Developed using the ECL technique.
Predicted band size: 92 kDa -
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab52031 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing Endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab52031 has been referenced in 1 publication.
- Salibe-Filho W et al. Shear stress-exposed pulmonary artery endothelial cells fail to upregulate HSP70 in chronic thromboembolic pulmonary hypertension. PLoS One 15:e0242960 (2020). PubMed: 33270690