Recombinant
RabMAb

Recombinant Anti-GRP94 antibody [EPR3988] - BSA and Azide free (ab238959)

Overview

  • Product name

    Anti-GRP94 antibody [EPR3988] - BSA and Azide free
    See all GRP94 primary antibodies
  • Description

    Rabbit monoclonal [EPR3988] to GRP94 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human GRP94 aa 650-750 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • IHC-P: Human hepatocellular carcinoma tissue.
  • General notes

    ab238959 is the carrier-free version of ab108606 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab238959 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab238959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 94 kDa (predicted molecular weight: 92 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Antigen retrieval is recommended.

ICC Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
    • Sequence similarities

      Belongs to the heat shock protein 90 family.
    • Cellular localization

      Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links

    • Alternative names

      • 94 kDa glucose regulated protein antibody
      • 94 kDa glucose-regulated protein antibody
      • ECGP antibody
      • Endoplasmin antibody
      • Endothelial cell (HBMEC) glycoprotein antibody
      • ENPL_HUMAN antibody
      • Glucose regulated protein 94kDa antibody
      • gp96 antibody
      • gp96 homolog antibody
      • GRP 94 antibody
      • GRP-94 antibody
      • Heat shock protein 90 kDa beta member 1 antibody
      • heat shock protein 90kDa beta (Grp94), member 1 antibody
      • Heat shock protein, 90 kDa, beta, 1 antibody
      • HSP90B1 antibody
      • Stress inducible tumor rejection antigen GP96 antibody
      • TRA1 antibody
      • tumor rejection antigen (gp96) 1 antibody
      • Tumor rejection antigen 1 antibody
      • Tumor rejection antigen gp96 antibody
      • Tumor rejection antigen-1 (gp96) antibody
      see all

    Images

    • Ab108606 staining GRP94 in paraffin embedded Mouse liver carcinoma tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin embedded sections). Samples are incubated in primary antibody at 1:1000 dilution (0.57µg/ml). A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Hematoxylin was used as a counterstain. Cytoplasmic staining on mouse liver.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108606).

    • Ab108606 staining GRP94 in paraffin embedded Human lung carcinoma tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin embedded sections). Samples are incubated in primary antibody at 1:1000 dilution (0.57µg/ml). A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Hematoxylin was used as a counterstain. Cytoplasmic staining on human lung carcinoma. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108606).

    • ab108606, at 1/100 dilution, staining GRP94 in paraffin-embedded Human breast tissue by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108606).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab108606, at 1/100 dilution, staining GRP94 in paraffin-embedded Human gastric adenocarcinoma tissue by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108606).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab108606 staining GRP94 in paraffin embedded human hepatocellular carcinoma tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin embedded sections).

      Samples are incubated in primary antibody at 1:1000 dilution (0.57 µg/ml). A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Hematoxylin was used as a counterstain. Cytoplasmic staining on human hepatocellular carcinoma.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab108606).

    References

    ab238959 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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