Anti-GRP94 antibody [SPM249], prediluted (ab54244)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-GRP94 antibody [SPM249], prediluted
See all GRP94 primary antibodies -
DescriptionRat monoclonal [SPM249] to GRP94, prediluted
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Host speciesRat
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Tested applicationsSuitable for: IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human, Monkey
Predicted to work with: Sheep, Rabbit, Horse, Chicken, Guinea pig, Hamster, Cow, Dog, Pig, Xenopus laevis
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Immunogen
Purified glucose regulated protein 94 (GRP94) from chicken oviducts.
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Positive control
- SKBR-3 cells and tonsil tissue.
Properties
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FormPrediluted
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: BSA, 0.0268% PBS -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone numberSPM249
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IsotypeIgG2a
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Light chain typekappa
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab54244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Application notes
IHC-P: Ready to use for 30 min at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionMolecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
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Sequence similaritiesBelongs to the heat shock protein 90 family.
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Cellular localizationEndoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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Database links
- Entrez Gene: 374163 Chicken
- Entrez Gene: 282646 Cow
- Entrez Gene: 100723767 Guinea pig
- Entrez Gene: 7184 Human
- Entrez Gene: 22027 Mouse
- Entrez Gene: 397191 Pig
- Entrez Gene: 100009456 Rabbit
- Entrez Gene: 362862 Rat
see all -
Alternative names
- 94 kDa glucose regulated protein antibody
- 94 kDa glucose-regulated protein antibody
- ECGP antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [SPM249], prediluted (ab54244)](https://www.abcam.com/ps/products/54/ab54244/Images/ab54244_1.bmp)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [SPM249], prediluted (ab54244)ab54244 staining human GRP94 in human tonsil tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.
Protocols
References
This product has been referenced in:
- Chen Y et al. Expression of heat-shock protein gp96 in gallbladder cancer and its prognostic clinical significance. Int J Clin Exp Pathol 8:1946-53 (2015). Read more (PubMed: 25973087) »
- Leemans JC et al. The role of Toll-like receptor 2 in inflammation and fibrosis during progressive renal injury. PLoS One 4:e5704 (2009). IHC-P ; Mouse . Read more (PubMed: 19479087) »
