Overview

  • Product name

    Grp94 ELISA Set (Reagents for 5 x 96 well plates, without plates)
  • Sample type

    Cell Lysate, Microsomes
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.29 ng/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse, Rat, Dog, Human
  • Product overview

    Abcam’s Grp94 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Grp94 in Biological fluids.

    A monoclonal antibody specific for Grp94 is coated onto 96-well plates. Samples and standards of Grp94 are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, Grp94 is recognized by the addition of a biotinylated monoclonal antibody specific for Grp94. After removal of excess biotinylated antibody, streptavidin-peroxidase is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’- tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of Grp94 in the samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

Applications

Our Abpromise guarantee covers the use of ab136950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab136950.

  • Concentrations of mouse Grp94 were measured in lysates from either control or heat-shocked 3T3 cells. Cell lysates were prepared using Extraction Reagent, and were serially diluted into assay buffer prior to the assay.

  • Dose-response curves from cell lysates diluted into assay buffer (using the MRD) were compared to the recombinant Canine Grp94 standard curve. Parallelism indicates antibody-binding characteristics of the native and standard proteins are similar, allowing accurate determination of analyte.

Protocols

References

ab136950 has not yet been referenced specifically in any publications.

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