Overview

  • Product name

  • Description

    Rabbit polyclonal to GRPR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human GRPR.

    Read Abcam's proprietary immunogen policy (Peptide available as ab39961.)

  • Positive control

    • Recombinant Human GRPR protein (ab114381) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: Human brain membrane lysate MDA MB 361 (human breast adenocarcinoma) whole cell lysate Caco2 (Human colonic carcinoma) whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab39883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 43 kDa).

Target

  • Function

    Receptor for gastrin releasing peptide (GRP). This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • Tissue specificity

    Highly expressed in pancreas. Also expressed in stomach, adrenal cortex and brain.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • BB2 antibody
    • Bombesin BB2 receptor antibody
    • bombesin receptor 2 antibody
    • Gastrin Releasing Peptide Receptor antibody
    • Gastrin-releasing peptide receptor antibody
    • GRP preferring bombesin receptor antibody
    • GRP R antibody
    • GRP-preferring bombesin receptor antibody
    • GRP-R antibody
    • Grp/bombesin receptor antibody
    • Grpr antibody
    • GRPR_HUMAN antibody
    see all

Images

  • All lanes : Anti-GRPR antibody (ab39883) at 1 µg/ml

    Lane 1 : Brain (Human) Membrane Lysate
    Lane 2 : MDA MB 361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : Caco 2 whole cell lysate (ab3950)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 53 kDa
    why is the actual band size different from the predicted?



    This protein is known to be glycosylated which could explain the difference between the predicted molecular weight of 43kDa according to Swiss Prot and the observed molecular weight of 53kDa.
  • ab39883 staining human pancreas tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retreival in citrate buffer (pH 6) prior to blocking in sequential (prediluted) peroxidase and protein block for 45 minutes at 20°C.  The primary antibody was diluted 1/100 and incubated with the sample for 45 minutes at 20°C.  A HRP conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

References

This product has been referenced in:

See all 4 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

Having reviewed this case, I would appreciate if you can confirm some further detailsto helptooptimise the results from ab39883 Anti-GRPR antibody:

1.This receptor is a multi-pass membrane protein, which can be a bit tricky sometimes. I am wondering about if you have used a lysis buffer in addition to the snap freezing? Did you boil the samples prior to loading to the gel? If so, at what temperature and under reducing conditions?

2. What is the "indicated solution" you use for blocking?

3. Do you add a detergent during primary AB incubation?

4.How long is the washing step after theprimary AB incubation?



In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

Read More

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab101937.

You can expect to receive this on Feb 7th. To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain

Dr. Sophie Pezet

Verified customer

Submitted Nov 22 2010

Application
Western blot
Sample
Human Purified protein (Caco-2 Cells)
Loading amount
200 µg
Specification
Caco-2 Cells
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12% acrylamide gel)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 10 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Tumor)
Specification
Tumor
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 10 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human (pancreas) mouse and rat (kidney))
Specification
human (pancreas) mouse and rat (kidney)
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Permeabilization
No
Blocking step
Sequential (prediluated) peroxidase and protein block as blocking agent for 45 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

Submitted Apr 04 2008

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