Overview

  • Product name

  • Description

    Rabbit polyclonal to GRPR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    TNPSVATFSLINGNICHERYV

    , corresponding to C terminal amino acids 364-384 of Human GRPR

  • Positive control

    • Recombinant Human GRPR protein (ab114381) can be used as a positive control in WB.

Properties

Applications

Our Abpromise guarantee covers the use of ab48357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.

Target

  • Function

    Receptor for gastrin releasing peptide (GRP). This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • Tissue specificity

    Highly expressed in pancreas. Also expressed in stomach, adrenal cortex and brain.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • BB2 antibody
    • Bombesin BB2 receptor antibody
    • bombesin receptor 2 antibody
    • Gastrin Releasing Peptide Receptor antibody
    • Gastrin-releasing peptide receptor antibody
    • GRP preferring bombesin receptor antibody
    • GRP R antibody
    • GRP-preferring bombesin receptor antibody
    • GRP-R antibody
    • Grp/bombesin receptor antibody
    • Grpr antibody
    • GRPR_HUMAN antibody
    see all

Images

  • Immunohistochemical staining of GRPR in paraffin embedded Human mammary carcinoma tissue sections using ab48357.

References

ab48357 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your patience in this matter. The originator of ab48357 (GRPR antibody) has informed us that this product was tested in WB using human breast carcinoma and pheochromocytoma tumor samples. I have included the original reference for this antibody as well as a summary of the Originator's Western blot protocol below. Reference: Schultz, Solveig, Rocken, C.; Schultz, S. Immunohistochemical detection of bombesin receptor subtypes GRP-R and BRS-3 in human tumors using novel anti-peptide antibodies Virchows Archiv, 449: 421-427, 2006 http://www.springerlink.com/content/fgn35730v17j3284/fulltext.pdf#http:/ /www.springerlink.com/content/fgn35730v17j3284/fulltext.pdf Western blot analysis: "Membranes were prepared from fresh tumor specimens. Tissues were lysed in homogenization buffer (5 mM EDTA, 3 mM EGTA, 250 mM sucrose, and 10 mM Tris-HCL at pH 7.6 containing 1 mM phenylmethylsulfonyl fluoride, 1 uM pepstatin A, 10 ug/ml leupeptin, and 2 ug/ml aprotinin), and membranes were pelleted at 20,000xg for 30 min. at 4 degrees C. Membranes were then dissolved in lysis buffer (150 mM NaCl, 5 mM, EDTA, 3 mM EGTA, 20 mM Hepes at pH 7.4 containing 4 mg/ml dodecyl-beta-maltoside and proteinase inhibitors as above) and incubated with 150 ul wheat germ lectin agarose beads (Amersham) for 90 min at 4 deg. C. Beads were washed five times in lysis buffer and adsorbed glycoproteins were eluted with sodium dodecyl sulfate (SDS) sample buffer for 20 min at 60 deg. C. Samples were then subjected to 8% SDS PAGE and immunobloted onto nitrocellulose. Blots were incubated with 1 ug/ml anti-GRP-R or 1 ug/ml anti-BRS-3 antibodies followed by peroxidase-conjugated secondary antibodies and enhanced chemiluminescence detection (Amersham). For adsorption controls, the antibody was preincubated with 10 ug/ml of its cognate peptide for 2 h at RT." I hope this information helps. Please don't hesitate to contact us again if you have any further questions.

Read More

Answer

Thank you for your enquiry. We do not have the purified protein but it is highly expressed in normal pancreas. I suggest our membrane lysate of human pancreas, ab29804: https://www.abcam.com/index.html?datasheet=29804 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up