Key features and details
- Rabbit polyclonal to GRSF1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GRSF1 antibody
See all GRSF1 primary antibodies
DescriptionRabbit polyclonal to GRSF1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human GRSF1 aa 325-375. The exact sequence is proprietary.
Database link: Q12849
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to GRSF1 immobilized on solid support
Our Abpromise guarantee covers the use of ab241399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 53 kDa.|
FunctionRegulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences.
Sequence similaritiesContains 3 RRM (RNA recognition motif) domains.
Cellular localizationCytoplasm. Mitochondrion matrix > mitochondrion nucleoid. Forms granules that colocalize with foci of newly synthesized mtRNA next to mitochondrial nucleoids.
- Information by UniProt
- G rich RNA sequence binding factor 1 antibody
- G rich sequence binding factor 1 antibody
- G-rich sequence factor 1 antibody
All lanes : Anti-GRSF1 antibody (ab241399) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 10 seconds
Lysates prepared in NETN buffer.
GRSF1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241399 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab241399 at 0.4 µg/mL.
Lane 1: ab241399 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241399 has not yet been referenced specifically in any publications.