Overview

  • Product name

  • Description

    Rabbit polyclonal to GRSF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human GRSF1 aa 100-150. The exact sequence is proprietary.
    Database link: Q12849

  • Positive control

    • IP: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab241400 was affinity purified using an epitope specific to GRSF1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Regulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences.
    • Sequence similarities

      Contains 3 RRM (RNA recognition motif) domains.
    • Cellular localization

      Cytoplasm. Mitochondrion matrix > mitochondrion nucleoid. Forms granules that colocalize with foci of newly synthesized mtRNA next to mitochondrial nucleoids.
    • Information by UniProt
    • Database links

    • Alternative names

      • G rich RNA sequence binding factor 1 antibody
      • G rich sequence binding factor 1 antibody
      • G-rich sequence factor 1 antibody
      • GRSF-1 antibody
      • GRSF1 antibody
      • GRSF1_HUMAN antibody
      see all

    Images

    • GRSF1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241400 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241400 at 1 µg/ml.

      Lane 1: ab241400 IP in HeLa whole cell lysate.
      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Detection: Chemiluminescence with exposure time of 30 seconds.

      Lysates prepared in NETN buffer.

    References

    ab241400 has not yet been referenced specifically in any publications.

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