Recombinant
RabMAb

Recombinant Anti-GRSF1 antibody [EPR16678] (ab205531)

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Overview

  • Product name

    Anti-GRSF1 antibody [EPR16678]
    See all GRSF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16678] to GRSF1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human GRSF1 aa 100-200. The exact sequence is proprietary.
    Database link: Q12849

  • Positive control

    • WB: HeLa, 293, HepG2, Jurkat, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; human fetal brain and fetal heart lysates; mouse brain and kidney lysates; rat brain and heart lysates. ICC/IF: HeLa and Jurkat cells. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16678

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab205531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
ICC/IF 1/250.
Flow Cyt 1/150.
IP 1/50.

Target

  • Function

    Regulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences.

  • Sequence similarities

    Contains 3 RRM (RNA recognition motif) domains.

  • Cellular localization

    Cytoplasm. Mitochondrion matrix > mitochondrion nucleoid. Forms granules that colocalize with foci of newly synthesized mtRNA next to mitochondrial nucleoids.
  • Information by UniProt

  • Database links

    • Alternative names

      • G rich RNA sequence binding factor 1 antibody
      • G rich sequence binding factor 1 antibody
      • G-rich sequence factor 1 antibody
      • GRSF-1 antibody
      • GRSF1 antibody
      • GRSF1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531)
      Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531)
      All lanes : Anti-GRSF1 antibody [EPR16678] (ab205531) at 1/1000 dilution

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
      Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 53 kDa
      Observed band size: 53 kDa


      Exposure time: 2 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-GRSF1 antibody [EPR16678] (ab205531)
      Immunocytochemistry/ Immunofluorescence - Anti-GRSF1 antibody [EPR16678] (ab205531)

      Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling GRSF1 with ab205531 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cell line.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab205531 at 1/250 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
      -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibodyat 1/1000 dilution.

    • Flow Cytometry - Anti-GRSF1 antibody [EPR16678] (ab205531)
      Flow Cytometry - Anti-GRSF1 antibody [EPR16678] (ab205531)

      Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling GRSF1 with ab205531 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

    • Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531)
      Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531)
      All lanes : Anti-GRSF1 antibody [EPR16678] (ab205531) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human fetal heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 53 kDa
      Observed band size: 53 kDa


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531)
      Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531)
      All lanes : Anti-GRSF1 antibody [EPR16678] (ab205531) at 1/1000 dilution

      Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
      Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus ) whole cell lysate
      Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
      Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 53 kDa
      Observed band size: 53 kDa


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531)
      Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531)
      All lanes : Anti-GRSF1 antibody [EPR16678] (ab205531) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Mouse kidney lysate
      Lane 3 : Rat brain lysate
      Lane 4 : Rat heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 53 kDa
      Observed band size: 53 kDa


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-GRSF1 antibody [EPR16678] (ab205531)
      Immunocytochemistry/ Immunofluorescence - Anti-GRSF1 antibody [EPR16678] (ab205531)

      Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling GRSF1 with ab205531 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab205531 at 1/250 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
      -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Immunoprecipitation - Anti-GRSF1 antibody [EPR16678] (ab205531)
      Immunoprecipitation - Anti-GRSF1 antibody [EPR16678] (ab205531)

      GRSF1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab205531 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab205531 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: HeLa whole cell lysate 10µg (Input).

      Lane 2: ab205531 IP in HeLa whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205531 in HeLa whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 5 seconds.

       

    References

    ab205531 has not yet been referenced specifically in any publications.

    Publishing research using ab205531? Please let us know so that we can cite the reference in this datasheet.

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