RecombinantRabMAb

Recombinant Anti-GRSF1 antibody [EPR16678] (ab205531)

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Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531)
  • Immunocytochemistry/ Immunofluorescence - Anti-GRSF1 antibody [EPR16678] (ab205531)
  • Flow Cytometry - Anti-GRSF1 antibody [EPR16678] (ab205531)
  • Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531)
  • Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531)
  • Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531)
  • Immunocytochemistry/ Immunofluorescence - Anti-GRSF1 antibody [EPR16678] (ab205531)
  • Immunoprecipitation - Anti-GRSF1 antibody [EPR16678] (ab205531)
  • Anti-GRSF1 antibody [EPR16678] (ab205531)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16678] to GRSF1
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GRSF1 antibody [EPR16678]
    See all GRSF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16678] to GRSF1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human GRSF1 aa 100-200. The exact sequence is proprietary.
    Database link: Q12849

  • Positive control

    • WB: HeLa, 293, HepG2, Jurkat, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; human fetal brain and fetal heart lysates; mouse brain and kidney lysates; rat brain and heart lysates. ICC/IF: HeLa and Jurkat cells. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16678

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab205531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
ICC/IF 1/250.
Flow Cyt 1/150.
IP 1/50.

Target

  • Function

    Regulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences.

  • Sequence similarities

    Contains 3 RRM (RNA recognition motif) domains.

  • Cellular localization

    Cytoplasm. Mitochondrion matrix > mitochondrion nucleoid. Forms granules that colocalize with foci of newly synthesized mtRNA next to mitochondrial nucleoids.
  • Information by UniProt

  • Database links

    • Alternative names

      • G rich RNA sequence binding factor 1 antibody
      • G rich sequence binding factor 1 antibody
      • G-rich sequence factor 1 antibody
      • GRSF-1 antibody
      • GRSF1 antibody
      • GRSF1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531)
      Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531)
      All lanes : Anti-GRSF1 antibody [EPR16678] (ab205531) at 1/1000 dilution

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
      Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 53 kDa
      Observed band size: 53 kDa


      Exposure time: 2 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-GRSF1 antibody [EPR16678] (ab205531)
      Immunocytochemistry/ Immunofluorescence - Anti-GRSF1 antibody [EPR16678] (ab205531)

      Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling GRSF1 with ab205531 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cell line.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab205531 at 1/250 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
      -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibodyat 1/1000 dilution.

    • Flow Cytometry - Anti-GRSF1 antibody [EPR16678] (ab205531)
      Flow Cytometry - Anti-GRSF1 antibody [EPR16678] (ab205531)

      Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling GRSF1 with ab205531 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

    • Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531)
      Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531)
      All lanes : Anti-GRSF1 antibody [EPR16678] (ab205531) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human fetal heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 53 kDa
      Observed band size: 53 kDa


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531)
      Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531)
      All lanes : Anti-GRSF1 antibody [EPR16678] (ab205531) at 1/1000 dilution

      Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
      Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus ) whole cell lysate
      Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
      Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 53 kDa
      Observed band size: 53 kDa


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531)
      Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531)
      All lanes : Anti-GRSF1 antibody [EPR16678] (ab205531) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Mouse kidney lysate
      Lane 3 : Rat brain lysate
      Lane 4 : Rat heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 53 kDa
      Observed band size: 53 kDa


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-GRSF1 antibody [EPR16678] (ab205531)
      Immunocytochemistry/ Immunofluorescence - Anti-GRSF1 antibody [EPR16678] (ab205531)

      Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling GRSF1 with ab205531 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab205531 at 1/250 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
      -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Immunoprecipitation - Anti-GRSF1 antibody [EPR16678] (ab205531)
      Immunoprecipitation - Anti-GRSF1 antibody [EPR16678] (ab205531)

      GRSF1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab205531 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab205531 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: HeLa whole cell lysate 10µg (Input).

      Lane 2: ab205531 IP in HeLa whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205531 in HeLa whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 5 seconds.

       

    • Anti-GRSF1 antibody [EPR16678] (ab205531)
      Anti-GRSF1 antibody [EPR16678] (ab205531)

    References (1)

    Publishing research using ab205531? Please let us know so that we can cite the reference in this datasheet.

    ab205531 has been referenced in 1 publication.

    • Xu C  et al. HnRNP F/H associate with hTERC and telomerase holoenzyme to modulate telomerase function and promote cell proliferation. Cell Death Differ 27:1998-2013 (2020). PubMed: 31863069

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