RecombinantRabMAb

Recombinant Anti-GRSF1 antibody [EPR16678] - BSA and Azide free (ab246330)

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Immunoprecipitation - Anti-GRSF1 antibody [EPR16678] - BSA and Azide free (ab246330)
  • Flow Cytometry - Anti-GRSF1 antibody [EPR16678] - BSA and Azide free (ab246330)
  • Immunocytochemistry/ Immunofluorescence - Anti-GRSF1 antibody [EPR16678] - BSA and Azide free (ab246330)
  • Immunocytochemistry/ Immunofluorescence - Anti-GRSF1 antibody [EPR16678] - BSA and Azide free (ab246330)
  • Anti-GRSF1 antibody [EPR16678] - BSA and Azide free (ab246330)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16678] to GRSF1 - BSA and Azide free
  • Suitable for: WB, IP, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GRSF1 antibody [EPR16678] - BSA and Azide free
    See all GRSF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16678] to GRSF1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human GRSF1 aa 100-200. The exact sequence is proprietary.
    Database link: Q12849

  • Positive control

    • ICC/IF: HeLa and Jurkat cells.Flow Cyt: HeLa cells.IP: HeLa whole cell lysate.

  • General notes

    Ab246330 is the carrier-free version of ab205531. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab246330 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16678

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab246330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Regulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences.

  • Sequence similarities

    Contains 3 RRM (RNA recognition motif) domains.

  • Cellular localization

    Cytoplasm. Mitochondrion matrix > mitochondrion nucleoid. Forms granules that colocalize with foci of newly synthesized mtRNA next to mitochondrial nucleoids.
  • Information by UniProt

  • Database links

    • Alternative names

      • G rich RNA sequence binding factor 1 antibody
      • G rich sequence binding factor 1 antibody
      • G-rich sequence factor 1 antibody
      • GRSF-1 antibody
      • GRSF1 antibody
      • GRSF1_HUMAN antibody
      see all

    Images

    • Immunoprecipitation - Anti-GRSF1 antibody [EPR16678] - BSA and Azide free (ab246330)
      Immunoprecipitation - Anti-GRSF1 antibody [EPR16678] - BSA and Azide free (ab246330)

      GRSF1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab205531 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab205531 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: HeLa whole cell lysate 10µg (Input).

      Lane 2: ab205531 IP in HeLa whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205531 in HeLa whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 5 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205531).

    • Flow Cytometry - Anti-GRSF1 antibody [EPR16678] - BSA and Azide free (ab246330)
      Flow Cytometry - Anti-GRSF1 antibody [EPR16678] - BSA and Azide free (ab246330)

      Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling GRSF1 with ab205531 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205531).

    • Immunocytochemistry/ Immunofluorescence - Anti-GRSF1 antibody [EPR16678] - BSA and Azide free (ab246330)
      Immunocytochemistry/ Immunofluorescence - Anti-GRSF1 antibody [EPR16678] - BSA and Azide free (ab246330)

      Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling GRSF1 with ab205531 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab205531 at 1/250 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
      -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205531).

    • Immunocytochemistry/ Immunofluorescence - Anti-GRSF1 antibody [EPR16678] - BSA and Azide free (ab246330)
      Immunocytochemistry/ Immunofluorescence - Anti-GRSF1 antibody [EPR16678] - BSA and Azide free (ab246330)

      Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling GRSF1 with ab205531 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cell line.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab205531 at 1/250 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
      -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibodyat 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205531).

    • Anti-GRSF1 antibody [EPR16678] - BSA and Azide free (ab246330)
      Anti-GRSF1 antibody [EPR16678] - BSA and Azide free (ab246330)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab246330? Please let us know so that we can cite the reference in this datasheet.

    ab246330 has been referenced in 1 publication.

    • Atashpaz S  et al. ATR expands embryonic stem cell fate potential in response to replication stress. Elife 9:N/A (2020). PubMed: 32163370

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