Overview

  • Product name

    Anti-GRSF1 antibody [EPR16679]
    See all GRSF1 primary antibodies
  • Description

    Rabbit monoclonal [EPR16679] to GRSF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human GRSF1 aa 350-450. The exact sequence is proprietary.
    Database link: Q12849

  • Positive control

    • HeLa, HepG2, 293 and Jurkat whole cell lysate (ab7899); Human liver tissue; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16679
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab194358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/250.
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
ICC/IF 1/100.

Target

  • Function

    Regulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences.
  • Sequence similarities

    Contains 3 RRM (RNA recognition motif) domains.
  • Cellular localization

    Cytoplasm. Mitochondrion matrix > mitochondrion nucleoid. Forms granules that colocalize with foci of newly synthesized mtRNA next to mitochondrial nucleoids.
  • Information by UniProt
  • Database links

  • Alternative names

    • G rich RNA sequence binding factor 1 antibody
    • G rich sequence binding factor 1 antibody
    • G-rich sequence factor 1 antibody
    • GRSF-1 antibody
    • GRSF1 antibody
    • GRSF1_HUMAN antibody
    see all

Images

  • All lanes : Anti-GRSF1 antibody [EPR16679] (ab194358) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling GRSF1 with ab194358 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling GRSF1 with ab194358 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).

  • Flow cytometry analysis of HeLa cells labelling GRSF1 (red) with purified ab194358 at dilution of 1/250. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

References

ab194358 has not yet been referenced specifically in any publications.

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