Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16679] to GRSF1
- Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
- Reacts with: Human
Product nameAnti-GRSF1 antibody [EPR16679]
See all GRSF1 primary antibodies
DescriptionRabbit monoclonal [EPR16679] to GRSF1
Tested applicationsSuitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide within Human GRSF1 aa 350-450. The exact sequence is proprietary.
Database link: Q12849
- HeLa, HepG2, 293 and Jurkat whole cell lysate (ab7899); Human liver tissue; HeLa cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab194358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).|
FunctionRegulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences.
Sequence similaritiesContains 3 RRM (RNA recognition motif) domains.
Cellular localizationCytoplasm. Mitochondrion matrix > mitochondrion nucleoid. Forms granules that colocalize with foci of newly synthesized mtRNA next to mitochondrial nucleoids.
- Information by UniProt
- G rich RNA sequence binding factor 1 antibody
- G rich sequence binding factor 1 antibody
- G-rich sequence factor 1 antibody
All lanes : Anti-GRSF1 antibody [EPR16679] (ab194358) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling GRSF1 with ab194358 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling GRSF1 with ab194358 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).
Flow cytometry analysis of HeLa cells labelling GRSF1 (red) with purified ab194358 at dilution of 1/250. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
ab194358 has not yet been referenced specifically in any publications.