Overview

  • Product name
  • Description
    Rabbit polyclonal to GRWD1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Common marmoset, Orangutan
  • Immunogen

    Synthetic peptide within Human GRWD1 aa 1-50. The exact sequence is proprietary. NP_113673.2
    Sequence:

    MAARKGRRRT CETGEPMEAE SGDTSSEGPA QVYLPGRGPP LREGEELVMD


    Database link: Q9BQ67

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 99% Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab176815 was affinity purified using an epitope specific to GRWD1 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • CDW4 antibody
    • CUL4 and DDB1 associated WDR protein 4 antibody
    • Glutamate rich WD repeat containing 1 antibody
    • Glutamate rich WD repeat containing protein 1 antibody
    • Glutamate rich WD repeat protein GRWD antibody
    • Glutamate-rich WD repeat-containing protein 1 antibody
    • GRWD 1 antibody
    • GRWD antibody
    • grwd1 antibody
    • GRWD1_HUMAN antibody
    • KIAA1942 antibody
    • METTL18 antibody
    • Regulator of ribosome biogenesis 1 homolog antibody
    • RRB 1 antibody
    • RRB1 antibody
    • WD repeat domain 28 antibody
    • WDR 28 antibody
    • WDR28 antibody
    see all

Images

  • All lanes : Anti-GRWD1 antibody (ab176815) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysates at 50 µg
    Lane 2 : HeLa whole cell lysates at 15 µg
    Lane 3 : HeLa whole cell lysates at 5 µg
    Lane 4 : 293T whole cell lysates at 50 µg

    Developed using the ECL technique.

    Predicted band size: 49 kDa


    Exposure time: 30 seconds
  • Detection of GRWD1 by Western Blot of Immunprecipitate.
    ab176815 at 0.1 µg/ml labeling GRWD1 in HeLa whole cell lysate immunoprecipitated using ab176815 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab176815 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab176815.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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