Key features and details
- Rabbit polyclonal to GRWD1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GRWD1 antibody
See all GRWD1 primary antibodies
DescriptionRabbit polyclonal to GRWD1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Common marmoset, Orangutan
- HeLa and 293T whole cell lysates.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab176815 was affinity purified using an epitope specific to GRWD1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab176815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 49 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
- Information by UniProt
- CDW4 antibody
- CUL4 and DDB1 associated WDR protein 4 antibody
- Glutamate rich WD repeat containing 1 antibody
All lanes : Anti-GRWD1 antibody (ab176815) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysates at 50 µg
Lane 2 : HeLa whole cell lysates at 15 µg
Lane 3 : HeLa whole cell lysates at 5 µg
Lane 4 : 293T whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 49 kDa
Exposure time: 30 seconds
Detection of GRWD1 by Western Blot of Immunprecipitate.
ab176815 at 0.1 µg/ml labeling GRWD1 in HeLa whole cell lysate immunoprecipitated using ab176815 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
ab176815 has not yet been referenced specifically in any publications.