Product nameAnti-GRWD1 antibody
See all GRWD1 primary antibodies
DescriptionRabbit polyclonal to GRWD1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human GRWD1 aa 350-400. The exact sequence is proprietary. (NP_113673.2).
Database link: Q9BQ67
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab224808 was affinity purified using an epitope specific to GRWD1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab224808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 49 kDa.|
- Information by UniProt
- CDW4 antibody
- CUL4 and DDB1 associated WDR protein 4 antibody
- Glutamate rich WD repeat containing 1 antibody
All lanes : Anti-GRWD1 antibody (ab224808) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 49 kDa
Exposure time: 30 seconds
GRWD1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab224808 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224808 at 0.1 µg/ml.
Lane 1: ab224808 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab224808 has not yet been referenced specifically in any publications.