Anti-GRX2 antibody (ab191292)
Key features and details
- Rabbit polyclonal to GRX2
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GRX2 antibody
See all GRX2 primary antibodies -
Description
Rabbit polyclonal to GRX2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Cow, Orangutan -
Immunogen
Synthetic peptide corresponding to Human GRX2 aa 100 to the C-terminus (internal sequence).
Database link: Q9NS18 -
Positive control
- Rat Testis Tissue Lysate; HeLa, U87, MCF7 and NEU cell lysates; Rat brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 18 kDa.
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 18 kDa. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release. -
Tissue specificity
Widely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta and lung. Not expressed in peripheral blood leukocytes. -
Sequence similarities
Belongs to the glutaredoxin family.
Contains 1 glutaredoxin domain. -
Cellular localization
Mitochondrion and Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 513762 Cow
- Entrez Gene: 51022 Human
- Entrez Gene: 69367 Mouse
- Entrez Gene: 114022 Rat
- Omim: 606820 Human
- SwissProt: Q32L67 Cow
- SwissProt: Q9NS18 Human
- SwissProt: Q923X4 Mouse
see all -
Alternative names
- bA101E13.1 (GRX2 glutaredoxin (thioltransferase) 2) antibody
- bA101E13.1 antibody
- CGI133 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling GRX2 with ab191292 at 1 μg/ml.
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All lanes : Anti-GRX2 antibody (ab191292) at 0.5 µg/ml
Lane 1 : Rat Testis Tissue Lysate
Lane 2 : HeLa cell Lysate
Lane 3 : U87 cell Lysate
Lane 4 : NEU cell Lysate
Lane 5 : Jurkat cell Lysate
Lane 6 : MCF7 cell Lysate
Predicted band size: 18 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab191292 has been referenced in 4 publications.
- Ahmed F et al. The effects of acute BPA exposure on skeletal muscle mitochondrial function and glucose metabolism. Mol Cell Endocrinol 499:110580 (2020). PubMed: 31536778
- Caron A et al. Effects of PCB126 on Adipose-to-Muscle Communication in an in Vitro Model. Environ Health Perspect 128:107002 (2020). PubMed: 33026256
- Tremblay-Laganière C et al. Polychlorinated biphenyl 126 exposure in rats alters skeletal muscle mitochondrial function. Environ Sci Pollut Res Int 26:2375-2386 (2019). PubMed: 30467749
- Liu X et al. Salvianolic Acid B (Sal B) Protects Retinal Pigment Epithelial Cells from Oxidative Stress-Induced Cell Death by Activating Glutaredoxin 1 (Grx1). Int J Mol Sci 17:N/A (2016). WB ; Human . PubMed: 27827892