Key features and details
- Rabbit polyclonal to GRX2
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-GRX2 antibody
See all GRX2 primary antibodies
DescriptionRabbit polyclonal to GRX2
SpecificityNo cross reactivity with other proteins.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow, Orangutan
- Rat Testis Tissue Lysate; HeLa, U87, MCF7 and NEU cell lysates; Rat brain tissue.
This product was previously labelled as Glutaredoxin 2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 18 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionGlutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release.
Tissue specificityWidely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta and lung. Not expressed in peripheral blood leukocytes.
Sequence similaritiesBelongs to the glutaredoxin family.
Contains 1 glutaredoxin domain.
Cellular localizationMitochondrion and Nucleus.
- Information by UniProt
- bA101E13.1 (GRX2 glutaredoxin (thioltransferase) 2) antibody
- bA101E13.1 antibody
- CGI133 antibody
Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling GRX2 with ab191292 at 1 μg/ml.
All lanes : Anti-GRX2 antibody (ab191292) at 0.5 µg/ml
Lane 1 : Rat Testis Tissue Lysate
Lane 2 : HeLa cell Lysate
Lane 3 : U87 cell Lysate
Lane 4 : NEU cell Lysate
Lane 5 : Jurkat cell Lysate
Lane 6 : MCF7 cell Lysate
Predicted band size: 18 kDa
ab191292 has been referenced in 2 publications.
- Tremblay-Laganière C et al. Polychlorinated biphenyl 126 exposure in rats alters skeletal muscle mitochondrial function. Environ Sci Pollut Res Int 26:2375-2386 (2019). PubMed: 30467749
- Liu X et al. Salvianolic Acid B (Sal B) Protects Retinal Pigment Epithelial Cells from Oxidative Stress-Induced Cell Death by Activating Glutaredoxin 1 (Grx1). Int J Mol Sci 17:N/A (2016). WB ; Human . PubMed: 27827892