Overview

  • Product name

  • Description

    Rabbit polyclonal to GRX2
  • Host species

    Rabbit
  • Specificity

    No cross reactivity with other proteins.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human GRX2 aa 103-119 (internal sequence).
    Sequence:

    EYGNQFQDALYKMTGER


    Database link: Q9NS18

  • Positive control

    • Rat Testis Tissue Lysate; HeLa, U87, MCF7 and NEU cell lysates; Rat brain tissue.
  • General notes

     This product was previously labelled as Glutaredoxin 2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab191292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 18 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release.
  • Tissue specificity

    Widely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta and lung. Not expressed in peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the glutaredoxin family.
    Contains 1 glutaredoxin domain.
  • Cellular localization

    Mitochondrion and Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • bA101E13.1 (GRX2 glutaredoxin (thioltransferase) 2) antibody
    • bA101E13.1 antibody
    • CGI133 antibody
    • Glrx2 antibody
    • GLRX2_HUMAN antibody
    • Glutaredoxin-2 antibody
    • Glutaredoxin-2, mitochondrial antibody
    • GRX2 antibody
    • mitochondrial antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling GRX2 with ab191292 at 1 μg/ml.

  • All lanes : Anti-GRX2 antibody (ab191292) at 0.5 µg/ml

    Lane 1 : Rat Testis Tissue Lysate
    Lane 2 : HeLa cell Lysate
    Lane 3 : U87 cell Lysate
    Lane 4 : NEU cell Lysate
    Lane 5 : Jurkat cell Lysate
    Lane 6 : MCF7 cell Lysate

    Predicted band size: 18 kDa

References

This product has been referenced in:

  • Tremblay-Laganière C  et al. Polychlorinated biphenyl 126 exposure in rats alters skeletal muscle mitochondrial function. Environ Sci Pollut Res Int 26:2375-2386 (2019). Read more (PubMed: 30467749) »
  • Liu X  et al. Salvianolic Acid B (Sal B) Protects Retinal Pigment Epithelial Cells from Oxidative Stress-Induced Cell Death by Activating Glutaredoxin 1 (Grx1). Int J Mol Sci 17:N/A (2016). WB ; Human . Read more (PubMed: 27827892) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191292.
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